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Five bacterial strains, which are able to grow and to disintegrate dibenzothiophene (DBT) and diben-zothiophene sulfone (DBTO2) in fossil fuels, are isolated. Analysis of products of DBT metabolized by these strains shows that different bacteria strains oxidize DBT by different pathways. The isolated strains R-6, R-16, R-9 and R-8 can metabolize DBT to DBTO2 and 2-hydroxybiphenyl (HBP), which are identified as Bacillus brevis, Bacillus sphaericus, Nocardia globerula and Pseudomonas delafieldii respectively. Another strain R-12 identified as Pseu-domonas sp. can degrade DBT completely but it cannot produce DBTO2 and HBP. The optimum temperature and initial pH for desulfurization by R-8 are 32℃ and 7.02 respectively and pH of the broth decreases during biodegradation. The growth of strain R-8 with different sulfur-sources indicates that this strain in DBT medium has an induction period of 3 days, which is longer than those with dimethylsulfoxide and MgSO4 media, but the growth rate of the bacterial strain
Five bacterial strains, which are able to grow and to disintegrate dibenzothiophene (DBT) and diben-zothiophene sulfone (DBTO2) in fossil fuels, are isolated. Analysis of products of DBT metabolized by these strains shows that different bacteria strain oxidize DBT by different pathways . The isolated R-6, R-16, R-9 and R-8 can metabolize DBT to DBTO2 and 2-hydroxybiphenyl (HBP), which are identified as Bacillus brevis, Bacillus sphaericus, Nocardia globerula and Pseudomonas delafieldii respectively. Another strain R-12 identified as Pseu-domonas sp. can degrade DBT completely but it can not produce DBTO2 and HBP. The optimum temperature and initial pH for desulfurization by R-8 are 32 ° C and 7.02 respectively and pH of the broth decreases during biodegradation. The growth of strain R-8 with different sulfur-sources that that strain in DBT medium has an induction period of 3 days, which is longer than those with dimethylsulfoxide and MgSO4 media, but the growth rate of the bacterial strain