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NAC转录因子在植物生长发育、信号传导和逆境胁迫应答方面起重要的作用。本研究根据芒果c DNA-SCo T差异显示中发现的c DNA片段设计特异引物,通过改良RACE技术得到了芒果NAC基因的全长c DNA序列。序列分析显示,该c DNA全长为1 167 bp,开放阅读框长度为1 002 bp,编码334个氨基酸,推测其分子量为37.41 k D,等电点为8.76,属于NAM基因家族,命名为Mi NAC。利用实时荧光定量技术对Mi NAC基因在芒果4℃低温胁迫、盐胁迫和PEG-6000胁迫后0、24 h、48 h和72 h时叶片和茎中表达模式进行分析,实验结果显示,这三种逆境处理均诱导芒果Mi NAC基因的表达,其中Mi NAC基因对4℃低温胁迫响应程度最高,其次是PEG胁迫和盐胁迫。结果表明,该基因参与了芒果逆境胁迫的分子调控。
NAC transcription factors play an important role in plant growth and development, signaling and stress response. In this study, specific primers were designed based on the c DNA fragments found in the differential display of DNA-SCo T in mango and the full-length c DNA sequence of mango NAC gene was obtained by modified RACE. Sequence analysis showed that the full-length cDNA of cDNA was 1 167 bp with an open reading frame of 1002 bp encoding a polypeptide of 334 amino acids with a predicted molecular mass of 37.41 kD and an isoelectric point of 8.76, which belonged to the NAM gene family named Mi NAC. The expression patterns of Mi NAC gene in leaves and stems at 4 ℃ low temperature stress, salt stress and PEG-6000 stress at 0, 24 h, 48 h and 72 h were analyzed by real-time fluorescence quantitative analysis. The experimental results showed that these three Mi NAC gene had the highest response to low temperature stress at 4 ℃, followed by PEG and salt stress. The results showed that the gene was involved in molecular regulation of stress in mango.