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苜蓿人工种子在60%RH中脱水3天,仍保持100%萌发和62.5%的成苗率,贮藏5天时失去发芽力。在45%RH中干燥3天后无萌发能力。非脱水状况下,人工种子保存在4℃中1个月,其发芽率和成苗率分别为91%和87%。人工种子直接播种于添加营养液的灭菌混合土中发芽率达100%,成苗率为41.6%。播种在无附加营养的混合土中虽然发芽率达100%,人工种子存活2个月以上,但不能转换成苗。人工种子在转换培养基上转化成苗后,移植至混合土中盆栽3个星期后幼苗成活率达80%以上。盆栽幼苗再移植至试验小区,成活率达98%以上。3个月后形成30cm高的致密草层,平均株高为48.8cm。
Alfalfa artificial seeds dehydrated in 60% RH for 3 days, still maintain 100% germination and 62.5% seedling rate, lose germination for 5 days when stored. No germination ability after drying in 45% RH for 3 days. Under non-dehydrated conditions, artificial seeds were stored at 4 ° C for 1 month and their germination rate and seedling formation rate were 91% and 87%, respectively. The germination rate of the artificial seeds directly sown in the sterilized mixed soil added with the nutrient solution was 100% and the seedling rate was 41.6%. Seeds planted in mixed soil with no added nutrients Although the germination rate of 100% artificial seeds survive more than 2 months, but can not be converted into seedlings. After the artificial seeds were transformed into seedlings on the transformation medium, the survival rate of the seedlings after transplanting to the mixed soil for 3 weeks was more than 80%. Potted seedlings and then transplanted to the test plot, the survival rate of 98% or more. After 3 months, a dense grass layer of 30 cm height was formed with an average plant height of 48.8 cm.