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目的通过检测妊娠期肝内胆汁淤积症(intrahepatic cholestasis of pregnancy,ICP)孕妇胎盘合体滋养细胞中白细胞介素18(IL-18)和半胱氨酸天冬氨酸蛋白酶-1(caspase-1)的定位和表达及caspase-1的生物学活性,探讨其与ICP发病的关系。方法 2011年3月至11月在重庆医科大学附属第一医院选择ICP孕妇40例为研究对象,包括ICP轻度组(20例),ICP重度组(20例),以同期行择期剖宫产术的20例正常孕妇为对照组。采用免疫组化检测IL-18和caspase-1的表达。结果 ICP重度组孕妇血清中IL-18及丙氨酸转氨酶、天冬氨酸转氨酶水平显著高于轻度组和正常对照组,差异有统计学意义(P<0.05)。ICP重度组胎盘合体滋养细胞中IL-18和caspase-1表达水平显著高于轻度组和正常对照组,差异有统计学意义(P<0.05)。结论 IL-18和caspase-1信号通路介导的免疫损伤可能参与了ICP孕妇的肝功能损害。
Objective To detect the levels of interleukin 18 (IL-18) and caspase-1 (IL-18) in placenta syncytiotrophoblasts in pregnant women with intrahepatic cholestasis of pregnancy (ICP) Localization and expression of caspase-1 and the biological activity of caspase-1, to explore its relationship with the incidence of ICP. Methods Forty cases of ICP pregnant women selected from the First Affiliated Hospital of Chongqing Medical University from March 2011 to November 2011 were enrolled in this study. Including ICP mild group (20 cases) and ICP severe group (20 cases), elective cesarean section Twenty normal pregnant women as control group. Immunohistochemistry was used to detect the expression of IL-18 and caspase-1. Results Serum levels of IL-18, alanine aminotransferase and aspartate aminotransferase in ICP severe group were significantly higher than those in mild group and normal control group (P <0.05). The levels of IL-18 and caspase-1 in placenta syncytiotrophoblast of ICP severe group were significantly higher than those in mild group and normal control group (P <0.05). Conclusion The immune injury mediated by IL-18 and caspase-1 signaling pathways may be involved in liver dysfunction in ICP pregnant women.