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目的探讨基质金属蛋白酶-9(MMP-9)和上皮型钙依赖黏附素(E-cadherin)在食管癌中的表达及其意义。方法应用免疫组化SP法检测69例食管癌组织及69例正常者胃镜取材组织中MMP-9和E-cadherin蛋白表达情况。结果食管鳞癌组织中MMP-9的阳性表达率高于正常食管黏膜鳞状上皮组织(71.0%vs46.4%,P<0.05),食管鳞癌组织中E-cadherin的阳性表达率低于正常食管黏膜鳞状上皮(50.7%vs72.5%,P<0.01)。MMP-9与E-cadherin的阳性表达率在食管癌不同病理分级及有无淋巴结转移中差异均有统计学意义(P<0.05或P<0.01);随着病理分级的增高及MMP-9阳性表达率依次增强,而E-cadherin阳性表达率依次降低(P<0.05或P<0.01);浸润达深肌层组E-cadherin蛋白阳性表达率低于浸润达浅肌层组(P<0.01);MMP-9与E-cadherin的蛋白表达呈负相关(r=-0.269,P<0.05)。结论 E-cadherin、MMP-9表达可能与食管癌的发生发展密切相关。
Objective To investigate the expression and significance of MMP-9 and E-cadherin in esophageal cancer. Methods The expression of MMP-9 and E-cadherin in 69 cases of esophageal carcinoma and 69 cases of normal gastroscopy tissue samples were detected by immunohistochemical SP method. Results The positive expression rate of MMP-9 in esophageal squamous cell carcinoma was significantly higher than that in normal esophageal squamous epithelium (71.0% vs46.4%, P <0.05). The positive rate of E-cadherin in esophageal squamous cell carcinoma was lower than that in normal esophageal squamous cell carcinoma Esophageal squamous epithelium (50.7% vs72.5%, P <0.01). The positive rates of MMP-9 and E-cadherin in esophageal cancer were significantly different between different pathological grade and lymph node metastasis (P <0.05 or P <0.01). With the increase of pathological grade and the positive rate of MMP-9 (P <0.05 or P <0.01). The positive expression rate of E-cadherin protein in deep submerged muscular layer group was lower than that in superficial muscular layer group (P <0.01) The protein expression of MMP-9 and E-cadherin was negatively correlated (r = -0.269, P <0.05). Conclusion The expression of E-cadherin and MMP-9 may be closely related to the occurrence and development of esophageal cancer.