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目的探讨急性白血病细胞的增殖活性及其在白血病预后判断上的作用。方法运用单克隆抗体免疫细胞化学染色技术检测了急性淋巴细胞白血病（ＡＬＬ）和急性非淋巴细胞白血病（ＡＮＬＬ）中增殖细胞核抗原（ＰＣＮＡ）的表达。结果ＰＣＮＡ在ＡＬＬ和ＡＮＬＬ中表达无显著性差异（Ｐ＞０．０５），在ＡＬＬ和ＡＮＬＬ中ＰＣＮＡ表达阳性细胞百分率分别为２８％～９５％和２６％～９６％。急性白血病中ＰＣＮＡ阳性细胞百分率与患者骨髓中幼稚细胞百分率显著相关（ｒ＝０．７６，Ｐ＜０．０１），与患者年龄、外周血白细胞计数、血红蛋白含量、血小板计数均无相关性。结论单克隆抗体免疫细胞化学方法可以很好地检测ＰＣＮＡ的表达，ＰＣＮＡ作为白血病细胞增殖活性的指标，其高表达可能是急性白血病预后不良的一个指标病细胞中表达要低得多，这可能是由于在体细胞受体内多种因素的影响，而使白血病细胞的增殖受到不同程度的限制，如能对这些因素作进一步研究，有可能找到一种治疗白血病甚至其它肿瘤的新方法。ＰＣＮＡ在急淋和急非淋中表达并无显著性差异，这与Ｋｅｉｍ等［２］对ＰＣＮＡ的研究结果不同，但Ｋｅｉｍ等是使用双向聚丙烯酰胺凝胶电泳检测ＰＣＮＡ在白血病细胞中的总体表达。这表明，在ＡＬＬ?
Objective To investigate the proliferative activity of acute leukemia cells and its role in the prognosis of leukemia. Methods The expression of proliferating cell nuclear antigen (PCNA) in acute lymphoblastic leukemia (ALL) and acute nonlymphocytic leukemia (ANLL) was detected by monoclonal antibody immunocytochemical staining. Results There was no significant difference in the expression of PCNA between ALL and ANLL (P>0.05). The percentage of PCNA-expressing cells in ALL and ANLL was 28%-95% and 26%-96%, respectively. The percentage of PCNA-positive cells in acute leukemia was significantly correlated with the percentage of immature cells in patients with bone marrow (r=0.76, P<0.01). No correlation was found between age, peripheral white blood cell count, hemoglobin, and platelet count. Conclusion The monoclonal antibody immunocytochemistry method can well detect the expression of PCNA. PCNA is an index of the proliferation activity of leukemia cells, and its high expression may be an indicator of poor prognosis of acute leukemia. The expression in diseased cells is much lower, which may be Due to various factors in somatic cell receptors, the proliferation of leukemic cells is restricted to different degrees. If these factors can be further studied, it is possible to find a new method for the treatment of leukemia or other tumors. There was no significant difference in the expression of PCNA in acute and acute non-lymphocytic cells. This is different from the results of Keim et al. [2] on PCNA, but Keim et al. used two-way polyacrylamide gel electrophoresis to detect the overall PCNA in leukemia cells. expression. This shows that in ALL?