论文部分内容阅读
目的建立西七骨伤片中柚皮苷的含量测定方法。方法采用高效液相色谱法,色谱柱:Phenomenex Luna C18柱(250 mm×4.6 mm,5μm);柱温:30℃;流动相:乙腈-0.05%冰醋酸溶液(20∶8 0);流速:1.0 mL.min?1;检测波长:283 nm。结果柚皮苷进样量在0.123 0～1.025 2μg内与峰面积有良好线性关系,平均加样回收率98.56%,RSD为0.90%(n=6)。结论该方法简便易行、准确可靠,可用于西七骨伤片的质量控制。
Objective To establish a method for the determination of naringin in xisqijiangyan tablets. Methods The column was Phenomenex Luna C18 (250 mm × 4.6 mm, 5 μm). The column temperature was 30 ℃. The mobile phase was acetonitrile-0.05% glacial acetic acid (20:80) 1.0 mL.min? 1; detection wavelength: 283 nm. Results The naringin injection had a good linear relationship with the peak area in the range of 0.123 0 ~ 1.025 2 μg with an average recovery of 98.56% and a RSD of 0.90% (n = 6). Conclusion The method is simple, accurate and reliable, and it can be used for the quality control of Xishenjianguang sheet.