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目的:建立三七接骨丸中三萜类皂苷(人参皂苷Rg1、人参皂苷Rb1、三七皂苷R1)的HPLC含量测定方法并考察其处方稳定性。方法:色谱柱:COSMOSIL 5 C18MS-II(250 mm×4.6 mm,5μm);流动相:水-乙腈,梯度洗脱;流速:1.0 ml·min-1;检测波长:203 nm,进样量10μl;柱温为30℃。同时考察在不同温度下制粒,三七中皂苷含量的变化。结果:三七皂苷R1、人参皂苷Rg1、和人参皂苷Rb1分别在0.211 8~2.648 0μg·ml-1(r=0.999 7),0.566 1~7.076 0μg·ml-1(r=0.999 7),0.317 2~3.964 5μg·ml-1(r=0.999 7)之间线性范围良好。平均回收率:三七皂苷R1为98.2%(RSD=0.82%,n=6),人参皂苷Rg1为98.1%(RSD=0.72%,n=6),人参皂苷Rb1为98.1%(RSD=0.59%,n=6)。三种皂苷随着温度增加降解也随之增加。结论:该方法简单、迅速,耐用,能够控制三七接骨丸的质量,同时建议三七接骨丸制粒干燥温度控制在60℃以下。
Objective: To establish a HPLC method for the determination of triterpene saponins (ginsenoside Rg1, ginsenoside Rb1, notoginsenoside R1) in Panax jiejiu pill and investigate its prescription stability. Method: Column: COSMOSIL 5 C18MS-II (250 mm × 4.6 mm, 5 μm); mobile phase: water-acetonitrile gradient elution; flow rate: 1.0 ml · min-1; The column temperature was 30 ℃. At the same time, we investigated the changes of saponin content in Panax notoginseng at different temperatures. Results: Notoginsenoside R1, ginsenoside Rg1, and ginsenoside Rb1 were detected in 0.211 8 ~ 2.648 μg · ml-1 (r = 0.999 7), 0.566 1 ~ 7.076 μg · ml-1 (r = 0.999 7) The linear range of 2 ~ 3.964 5μg · ml-1 (r = 0.999 7) was good. The average recovery was 98.2% (RSD = 0.82%, n = 6) for notoginsenoside R1, 98.1% for ginsenoside Rg1 (RSD = 0.72%, n = 6) and 98.1% for ginsenoside Rb1 (RSD = 0.59% , n = 6). Three kinds of saponins with increasing temperature degradation also increases. Conclusion: The method is simple, rapid and durable, which can control the quality of Sanqi jiegu pill. Meanwhile, it is suggested that the granulation drying temperature of Sanqi jiegu pill should be below 60 ℃.