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目的 通过动物实验观察甘露醇对肢体缺血再灌注所致肠损伤的保护作用。方法 80只Wistar大白鼠随机分成正常对照组 ,缺血 3小时和缺血 3小时用甘露醇 3个组。实验组于缺血、再灌注 1、2、3和 2 4小时取材光镜对比观察和组织学评估。结果 肢体缺血和再灌注后 ,实验组肠粘膜固有层毛细血管扩张、充血、大量多核白细胞浸润。大肠和小肠粘膜厚度和隐窝深度明显减少 (P <0 0 1) ,小肠绒毛高度相对增加 (P <0 0 1) ,隐窝细胞有变性坏死 ,用甘露醇组的病变明显轻于未用甘露醇组。结论 甘露醇能有效地减轻肢体缺血再灌注所致的肠损伤。
Objective To observe the protective effect of mannitol on intestinal injury induced by limb ischemia-reperfusion through animal experiments. Methods Eighty Wistar rats were randomly divided into normal control group, 3 hours ischemia and 3 hours ischemia with 3 groups of mannitol. The experimental group was observed under light microscope and histological evaluation at 1, 2, 3 and 24 hours after reperfusion. Results After limb ischemia and reperfusion, the experimental group of intestinal mucosa lamina propria capillaries dilate, hyperemia, a large number of polymorphonuclear leukocyte infiltration. The thickness of colorectal and intestinal mucosa and crypt depth were significantly decreased (P <0.01), the villus height was relatively increased (P <0.01), crypt cells were degenerated and necrotic, and the lesions in mannitol group were obviously lighter than those in unused Mannitol group. Conclusion Mannitol can effectively relieve the intestinal damage caused by limb ischemia-reperfusion.