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茉莉酸甲酯是一种调节植物形态发生、诱导防御相关基因的植物信号转导分子。为了解内源茉莉酸甲酯在植物发育中的作用,将编码茉莉酸甲基转移酶的NTR1基因与CaMV 35S启动子连接并导入大豆植株。PCR及Northern杂交结果表明,NTR1基因稳定整合在大豆基因组并得到过量表达。与野生型植株相比,转基因大豆叶片与根的形态发生了显著的变化。大部分转基因大豆叶片变得细长,初生根生长受到抑制而侧根的生长却受到促进。定量分析结果表明,转基因大豆植株叶片中茉莉酸甲酯的含量比对照高出 2～2.5 倍。这些结果表明,内源茉莉酸甲酯的积累参与了大豆形态发生的调控。
Methyl jasmonate is a plant signal transduction molecule that regulates plant morphogenesis and induces defense-related genes. To understand the role of endogenous methyl jasmonate in plant development, the NTR1 gene encoding jasmonic acid methyltransferase was linked to the CaMV 35S promoter and introduced into soybean plants. PCR and Northern blot results showed that the NTR1 gene was stably integrated in the soybean genome and overexpressed. Compared with the wild-type plants, the morphology of the leaves and roots of transgenic soybean changed significantly. Most of the transgenic soybean leaves became slender, with primary root growth inhibited and lateral root growth promoted. Quantitative analysis showed that the content of methyl jasmonate in transgenic soybean leaves was 2 ~ 2.5 times higher than that of the control. These results indicate that the accumulation of endogenous methyl jasmonate is involved in the regulation of soybean morphogenesis.