论文部分内容阅读
羊水甲胎蛋白(AFP)的测定已广泛用于早期产前诊断,以检出胎儿神精管缺损等疾病.虽然它有较高的敏感度和专一性,但单凭这一诊断,在临床上还是难以作出是否中断妊娠的决定.最近已经证明,羊水凝胶电泳和胆硷酯酶专一性染色相结合,可以作为产前诊断神精管缺损的一个有价值的手段.正常妇女的羊水在电泳后只呈现一条移动得很慢的胆硷酯酶区带,而在无脑儿或脊柱裂畸胎中,会出现另一条移动得很快的区带,使用选择性抑制剂可以证明这一增加的区带是乙酰胆硷酯酶(AChE).所有的羊水都在妊娠16～22周时抽取,AFP浓度用火箭免疫电泳测定,羊水贮存在零下20℃长达三年或反复冻融对AChE的测定结果都没有影响.
Determination of amniotic fluid alpha-fetoprotein (AFP) has been widely used in early prenatal diagnosis to detect fetal deformity and other diseases, although it has a high sensitivity and specificity, but this diagnosis alone Clinically it is still difficult to make the decision whether to interrupt the pregnancy.It has been recently demonstrated that amniotic gel electrophoresis and cholinesterase-specific staining combined can be used as a prenatal diagnosis of the defect of the seminiferous tubal a valuable means of normal women Amniotic fluid showed only a slow moving cholinesterase zone after electrophoresis, whereas in anencephalic or spina bifida there was another fast moving zone. Selective inhibitors were used to demonstrate The increase in this zone is acetylcholinesterase (AChE). All amniotic fluid is taken at 16-22 weeks gestation, and the AFP concentration is determined by rocket immunoelectrophoresis. Amniotic fluid is stored at -20 ° C for up to three years or repeatedly frozen Melting on AChE measurement results have no effect.