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目的探讨大鼠脂肪基质干细胞(ASCs)移植于梗死心肌后的增殖分化情况及对心功能的影响。方法培养大鼠ASCs,流式细胞术鉴定其表型。将SD大鼠左前降支结扎制造急性心肌梗死模型后,在梗死心肌处植入DAPI标记ASCs(实验组)或DMEM培养液(对照组)。移植后1及4周,超声检查心功能。并取梗死区心肌组织进行移植细胞形态学检查及毛细血管密度测定。RTPCR、ELISA法测梗死区VEGF的mRNA及蛋白表达情况。结果培养获得ASCs,表型为CD44+、CD105+,CD31-、CD45-。植入梗死区的ASCs可以分化为血管内皮细胞,实验组梗死心肌处血管密度较对照组明显增高(P<0.01),且VEGF基因及蛋白水平在移植后1周均较对照组明显增高(P<0.01)。移植后4周,实验组大鼠左室射血分数(LVEF)及左室短轴缩短率(FS)较对照组明显提高(P<0.01)。结论ASCs移植可以促进大鼠梗死后心肌血管新生,改善心功能。
Objective To investigate the proliferation and differentiation of rat adipose-derived stem cells (ASCs) transplanted to the infarcted myocardium and their effects on cardiac function. Methods Rat ASCs were cultured and their phenotypes were identified by flow cytometry. DAPI-labeled ASCs (experimental group) or DMEM culture medium (control group) were implanted into infarct myocardium after ligating left anterior descending artery of SD rats to establish acute myocardial infarction model. At 1 and 4 weeks after transplantation, cardiac function was examined by ultrasonography. Myocardial infarction and myocardial cell morphology and capillary density examination. RTPCR, ELISA method measured infarct VEGF mRNA and protein expression. Results ASCs were cultured and the phenotypes were CD44 +, CD105 +, CD31- and CD45-. ASCs implanted into the infarct area could differentiate into vascular endothelial cells. The density of vascular in infarction myocardium in experimental group was significantly higher than that in control group (P <0.01), and VEGF gene and protein level in control group were significantly increased at 1 week after transplantation <0.01). Four weeks after transplantation, left ventricular ejection fraction (LVEF) and left ventricular fractional shortening (FS) in experimental group were significantly higher than those in control group (P <0.01). Conclusion Transplantation of ASCs can promote myocardial angiogenesis after myocardial infarction and improve cardiac function.