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目的 研究大鼠生后发育期惊厥对其成年后再次惊厥所致神经元损伤的影响。方法 选择生后早期 (15d)高热致惊厥、成年后 (生后 5 5d)再次卡因酸 (KA)致惊厥大鼠 (FK组 )模型 ,与单纯成年期卡因酸致惊厥大鼠 (K组 )比较 ,观察成年期惊厥后大鼠的学习、记忆、行为学改变 ,海马苔藓纤维发芽以及海马凋亡细胞数的变化。结果 成年期大鼠惊厥后 ,FK及K组大鼠惊厥发作的潜伏期差异无显著性 [(4 0± 6 )s与 (4 6± 6 )s,P >0 0 5 ]。Morris水迷宫测试平均寻找平台时间 ,FK组[(2 4 3± 2 7)s]、K组 [(16 9± 3 3)s]时间较单纯热性惊厥组 (F组 ) [(6 0± 2 0 )s]、对照组 (C组 )[(5 5± 2 3)s]明显延长 (P <0 0 1) ,而且FK组也明显长于K组 (P <0 0 1) ,F组与C组差异无显著性(P >0 0 5 )。海马齿状回颗粒细胞上层Timm染色强度 ,FK组 (中位数为 2 83)和K组 (中位数为 2 33)较F组 (中位数为 0 2 5 )明显增强 (P <0 0 1) ,而FK组明显强于K组 (P <0 0 5 )。成年期应用KA后3d ,FK组、K组大鼠海马凋亡细胞数 (中位数分别为 2 1 9、14 5 )较F组 (中位数为 0 7)大鼠明显增多(P <0 0 1) ,同时FK组凋亡细胞数显著多于K组 (P <0 0 1)。结论 尽管发育期大鼠热性惊厥不能导致海马神经元凋亡 ,但在第 2次接受
Objective To study the effects of seizures during the developmental period of rats on the neuronal injury induced by recurrent seizures in adulthood. Methods Convulsions induced by hyperthermia in the early postnatal period (15 days) and reperfusion in rats (FK group) were induced by kainate (KA) after adulthood (postnatal days 5 and 5) Group) to observe the changes of learning, memory, behavior, hippocampal mossy fiber germination and hippocampal apoptotic cells in adulthood after convulsion. Results There was no significant difference in the latency of seizure between FK and K rats after convulsion in adult rats [(40 ± 6) s and (46 ± 6) s, P> 0.05). Morris water maze test averagely looked for plateau time in FK group [(2443 ± 27) s], K group [(16 9 ± 3 3) s] compared with simple febrile seizure group (F (P <0.01), but also significantly longer in FK group than in K group (P <0.01), F There was no significant difference between group and group C (P> 0.05). The intensity of Timm staining in the upper layer of granular cells in dentate gyrus of hippocampus was significantly higher in FK group (median 2 83) and K group (median 2 33) than in group F (median 0 25) (P <0 0 1), while the FK group was significantly stronger than the K group (P <0 05). The number of hippocampal apoptotic cells in FK group and K group was significantly higher than that in F group (median, 7 7, P < The number of apoptotic cells in FK group was significantly more than that in K group (P <0.01). Conclusions Although heat-induced seizures in developing rats fail to induce apoptosis in hippocampal neurons,