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利用兴安落叶松休眠顶芽作外植体,对4—30年生优良个体进行了离体培养,取得了丛生腋芽分化和生根的结果,分化率为60％—65％。其中30年生成年落叶松分化率仍可达60％,一个芽一次分化培养最多可产生26个丛生新芽,经分割培养多数能抽成新枝,经生根培养少部分生了根,形成完整植株。基本培养基以MS和SH较好,WPM次之。激素组合以BA1+NAA0.01和BA2+NAA0.2(单位:mg/l)分化效果最好。较好的培养程序为:①将芽接种于无激素固体培养基上先生长1—2周。②转入分化培养基进行分化培养2周。③在无激素培养基上继代培养直至分化出芽并开始抽枝。④分割成数丛继续培养抽枝直至枝高达到1.5cm以上。⑤从基部切下新枝扦插生根。⑥移栽入土。
Using the dormant buds of Larix gmelinii as explants, the fine individuals aged 4 to 30 years were cultured in vitro and the axillary bud differentiation and rooting results were obtained. The rate of differentiation was 60% -65%. Among them, the differentiation rate of larch can reach 60% at the age of 30 years. The maximum of 26 new shoots can be produced when a bud is differentiated and cultured. Most of the new shoots can be drawn into new shoots. The basic medium with MS and SH is better, followed by WPM. The best combination of hormones was BA1 + NAA0.01 and BA2 + NAA0.2 (unit: mg / l). The better culture program is as follows: ① The buds are inoculated on hormone-free solid medium for 1-2 weeks. ② into differentiation medium for differentiation and culture for 2 weeks. ③ subculture in hormone-free medium until differentiation buds and began to shoot. ④ divided into a few plexus continue to train until the branches up to 1.5cm above. ⑤ cut from the base of new cuttings rooting. ⑥ transplanted into the soil.