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目的观察光化学损伤大鼠单侧运动皮质建立后肢痉挛性偏瘫模型的可行性。方法 20只大鼠随机分为A、B两组。A组大鼠注射光敏性化学物质赤藓红B后,激光照射损伤左侧大脑运动皮质,B组大鼠不损伤皮质。分别以术前及术后3、7、14、28d的H反射频率依赖性抑制(RDD)来判断两组大鼠双侧后肢肌肉痉挛情况。术后28d以霍乱毒素行脊髓前角运动神经元逆行示踪及囊泡型谷氨酸转运体1(VGLUT1)免疫荧光染色。同时取大脑组织切片行H-E染色观察脑损伤部位的组织病理学改变。结果在术后3、7、14和28d,A组大鼠右后肢跖肌H反射RDD较B组右后肢减弱(P<0.01),A、B两组左后肢跖肌H反射RDD差异无统计学意义(P>0.05)。A组脊髓前角运动神经元胞体及突起上VGLUT1的数量较B组增加(P<0.01)。脑组织H-E染色可见A组大鼠左侧大脑皮质缺损,而B组无明显损伤。结论损伤大鼠单侧运动皮质,可造成对侧后肢痉挛性偏瘫。
Objective To observe the feasibility of establishing a hindlimb spastic hemiplegia model in unilateral motor cortex of photochemical damage rats. Methods Twenty rats were randomly divided into A and B groups. A group of rats injected with a photosensitive chemical substance Erythrosin B, the left cerebral cortex was injured by laser irradiation, and the rats in group B did not damage the cortex. The hypothalamic muscle spasm of the two groups were evaluated preoperatively and at 3, 7, 14 and 28 days after surgery respectively. At 28 days after operation, cholera toxin was used to retrograde tracing of anterior horn motor neurons in the spinal cord and immunofluorescent staining of vesicular glutamate transporter 1 (VGLUT1). At the same time take the brain tissue sections H-E staining to observe the histopathological changes of brain injury site. Results On the 3rd, 7th, 14th and 28th day after operation, the RD of the H plantar muscle of the right hindlimb in group A was weaker than that of the right hind limb in group B (P <0.01). There was no statistical difference in RD of plantar muscle between groups A and B Significance (P> 0.05). Compared with group B, the number of VGLUT1 in cell bodies and protrusions of motor neurons in group A was increased (P <0.01). Brain tissue H-E staining showed left cerebral cortex defect in group A, but no obvious damage in group B. Conclusion Injury rat unilateral motor cortex, can cause contralateral hind limb spastic hemiplegia.