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本研究探讨人骨髓间充质干细胞(MSC)对辐射损伤小鼠造血器官的保护作用。培养人骨髓MSC,并进行细胞学鉴定。BALB/c雌性小鼠经6 Gy60Coγ射线照射后,分别通过尾静脉注射不同剂量的人MSC、MSC裂解物或等体积生理盐水。每天记录小鼠存活情况,并于处理后的第9天和16天取小鼠股骨和脾脏进行病理学分析,观察造血组织容量,并进行细胞增生程度评分。结果表明,与对照组比较,MSC及细胞裂解物处理组小鼠生存期无明显延长,但第9天高剂量(5×106)MSC及裂解物组小鼠骨髓造血即部分恢复,造血组织容量显著改善,(43.50±12.08)%和(42.80±13.11)%vs(24.33±9.50)%(P<0.05),增生程度积分明显增高(P<0.05),脾脏出现增生结节。第16天高剂量MSC和裂解物处理组小鼠骨髓和脾脏细胞明显活跃,脾脏和骨髓结构接近正常小鼠。此外,两个时间点MSC与细胞裂解物处理组小鼠骨髓增生积分无明显差异。结论:骨髓MSC可促进辐射损伤小鼠的造血功能恢复,这种作用可能与细胞内固有的活性物质有关。
This study was to investigate the protective effect of human bone marrow mesenchymal stem cells (MSCs) on the hematopoietic organs of mice with radiation injury. Human bone marrow MSCs were cultured and identified by cytology. BALB / c female mice were irradiated with 6 Gy of 60Coγ ray, and different doses of human MSC, MSCs lysate or equal volume of saline were injected through tail vein. The survival of mice was recorded daily. Pathological analysis was performed on the femurs and spleens of mice on the 9th and 16th day after treatment. The hematopoietic tissue volume was observed and the degree of cell proliferation was scored. The results showed that compared with the control group, the survival time of the mice treated with MSC and cell lysate was not significantly prolonged, but the bone marrow hematopoiesis recovered partially in high dose (5 × 106) MSC and lysate group on the 9th day, and the hematopoietic tissue capacity (43.50 ± 12.08)% and (42.80 ± 13.11)% vs (24.33 ± 9.50)%, respectively (P <0.05). The integral of hyperplasia was significantly increased (P <0.05), and the proliferative nodules were found in the spleen. On the 16th day, the bone marrow and spleen cells of mice treated with high dose of MSCs and lysate were obviously active, and the structure of spleen and bone marrow was close to the normal mice. In addition, there was no significant difference in the myeloid hyperplastic score between MSCs and cell lysate-treated mice at two time points. Conclusion: Bone marrow MSCs can promote the recovery of hematopoietic function in mice with radiation injury, which may be related to the intrinsic active substances in the cells.