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目的 :探讨神经细胞粘附分子 ( neural cell adhesion molecule,NCAM)对人脑胶质瘤细胞株 U2 51 MG增殖行为的影响。 方法 :将外源性 NCAM-1 40质粒转入 U2 51 MG细胞株 ,采用流式细胞仪及培养细胞增殖动力学方法比较转染前后细胞的细胞周期、增殖细胞核抗原 ( proliferating cell nuclear antigen,PCNA)表达及群体增殖速度 ;在裸鼠颅内原位接种上述细胞 ,观察成瘤率及肿瘤大小。 结果 :NCAM转染后细胞的体外增殖速度减慢 ( P<0 .0 5) ,S期细胞减少 ,PCNA表达率下降 ;裸鼠颅内接种的成瘤率显著降低 ( P<0 .0 1 ) ,肿瘤体积明显较小。 结论 :转染外源性 NCAM在体外、体内均可抑制人脑胶质瘤细胞株 U2 51 MG的增殖。
Objective: To investigate the effect of neural cell adhesion molecule (NCAM) on the proliferation of human glioma cell line U2 51 MG. METHODS: The exogenous NCAM-1 40 plasmid was transferred into U2 51 MG cell line. The cell cycle and proliferating cell nuclear antigen (PCNA) of the cells before and after transfection were compared by flow cytometry and cultured cell proliferation kinetics. Expression and population proliferation rate; The above cells were inoculated intracranially in nude mice and the tumor formation rate and tumor size were observed. RESULTS: After NCAM transfection, the proliferation rate of the cells was slowed down in vitro (P<0.05), S phase cells were decreased, and the expression rate of PCNA was decreased; the tumorigenic rate of intracranial inoculation in nude mice was significantly reduced (P<0. 01). ), the tumor volume is significantly smaller. CONCLUSION: Transfection of exogenous NCAM can inhibit the proliferation of human glioma cell line U2 51 MG both in vitro and in vivo.