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目的探讨甘草酸二胺对骨髓间充质干细胞(MSC)移植治疗博来霉素(BLM)所致的大鼠肺纤维化的干预作用。方法造模并移入MSC后,腹腔注射甘草酸二胺28d,测定相关生化指标,PCR检测移植MSC向肺脏的迁移、免疫荧光染色观察肺泡Ⅱ型细胞(ATⅡ)的数量。结果与MSC组相比,甘草酸二胺+MSC组(GMSC组)大鼠肺组织羟脯氨酸(HPY)和血清MDA含量降低,而超氧化物歧化酶(SOD)活力升高。sry基因表达明显增多(P<0.01),肺组织ATⅡ增多。结论甘草酸二胺对MSC治疗肺纤维化有协同作用,其机制可能与机体内的抗氧化和促进MSC向肺组织的移入和向ATⅡ的转化有关。
Objective To investigate the intervention of glycyrrhizic acid diamine in the treatment of bleomycin (BLM) -induced pulmonary fibrosis induced by bone marrow mesenchymal stem cells (MSCs) in rats. Methods After modeling and transplanted into MSC, intraperitoneal injection of glycyrrhizic acid diamine for 28 days was used to measure the biochemical parameters. The migration of MSC transplanted to the lungs was detected by PCR. The number of type Ⅱ cells (AT Ⅱ) was observed by immunofluorescence staining. Results Compared with the MSC group, the content of hydroxyproline (HPY) and serum MDA in the lung tissue decreased and the activity of superoxide dismutase (SOD) increased in the glycyrrhizic acid diamine + MSC group (GMSC group). sry gene expression was significantly increased (P <0.01), AT II increased lung tissue. Conclusion Glycyrrhizic acid diamine has a synergistic effect on the treatment of pulmonary fibrosis with MSC. The mechanism may be related to the anti-oxidation and promoting the migration of MSC to lung tissue and the transformation to AT Ⅱ.