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目的 探讨雄激素受体 (AR)基因CAG微卫星多态性与前列腺癌 (PC)生物学行为的关系。方法 采用聚合酶链反应 单链构象多态性分析法 (PCR SSCP) ,对 37例中国人、5 7例美国白人以及 1 9例美国黑人PC患者共 1 1 3例PC标本 ,行AR基因CAG微卫星数量测定 ,并进行不同病理期别、细胞分化级别以及种族间的比较。结果 PC病变C期和D期的AR基因CAG数量均少于B期 ,其均数分别为 :中国人 2 2 .1 4± 2 .6 4与 2 5 .0 4± 1 .88(P <0 .0 1 )、美国白人 2 1 .97± 3.31与 2 3.74± 2 .49(P <0 .0 5 )、美国黑人 1 9.46± 3.1 4与 2 1 .75± 1 .1 0 (P <0 .0 5 ) ,差异显著。短的AR基因CAG (数量 <2 2 )的分布分别是 :中国人B期病变占1 3.0 % (3/2 3)、C～D期 2 8.6 % (4/1 4) ,美国白人B期 1 4.8% (4/2 7)、C～D期 36 .7% (1 1 /30 ) ,美国黑人B期 2 5 .0 % (1 /4 )、C～D期 80 .0 % (1 2 /1 5 ) ;其长的AR基因CAG(数量≥ 2 2 )的分布比例与之相反 ,差异显著 (P <0 .0 5 )。AR基因CAG数量随着肿瘤细胞分化程度的降低而减少 ,在高、中、低分化PC中其均数分别为 :中国人 2 5 .6 0± 1 .6 7、2 4.2 9± 3.45、2 3.6 4± 2 .5 6 ;美国白人 2 3.81± 2 .81、2 3.1 1± 1 .79、2 1 .44± 4.0 3;美国黑人 2 1 .0 0± 0 .0 0
Objective To investigate the relationship between the CAG microsatellite polymorphism of androgen receptor (AR) gene and the biological behavior of prostate cancer (PC). Methods A total of 113 PC specimens from 37 Chinese, 57 American white and 19 American black PC were detected by polymerase chain reaction (PCR) single strand conformation polymorphism (PCR SSCP). The AR gene CAG The number of microsatellites was measured, and different pathological stages, cell differentiation levels and inter-ethnic comparison were performed. Results The number of AR gene CAG in stage C and D of PC was less than that in stage B, and the mean numbers of AR were 22.14 ± 2.64 and 25.0 ± 0.88, respectively (P < 0. 01), American White 21.77 ± 3.31 and 2 3.74 ± 2.49 (P <0.05), African American 1 9.46 ± 3.1 4 and 2 1 .75 ± 1. 1 0 (P < 0 .0 5), significant difference. The distributions of short AR gene CAGs (<2 2) were as follows: Chinese B stage disease accounted for 13.0% (3/2 3), C ~ D stage 2 8.6% (4/1 4), white American B stage 1, 4.8% (4/2 7), 36.7% (11/30) in C ~ D, 25.0% (1/4) in African American B, 80.0% 2/1 5). The proportion of long AR gene CAG (number ≥ 2 2) was the opposite, with significant difference (P <0.05). The number of AR gene CAG decreased with the degree of differentiation of tumor cells reduced in high, moderate and poorly differentiated PC were: Chinese 2.56 ± 1 .6 7,2 4.2 9 ± 3.45,2 3.6 4 ± 2.56; American White 2 3.81 ± 2 .81,2 3.1 1 ± 1.79,2 1 .44 ± 4.0 3; African American 2 1 .0 0 ± 0 .0 0