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正常妊娠时尽管胎儿与母体间有抗原性差异,但不受排斥。其中蜕膜组织的作用似有特殊意义。至今关于蜕膜组织的报导不多,可能与标本难得有关。本文作者由蜕膜组织制备细胞悬液,检测细胞性质,并探讨其在妊娠前三个月中可能发生的免疫作用。自孕龄5—12周孕妇流产治疗中刮宫获得蜕膜组织。去除绒毛及其它组织,经细胞分离液离心:取界面细胞,染色检测细胞活性及形态。用正常志愿者或孕妇血单核细胞(MNC)作应答或刺激细胞,进行混合淋巴细胞反应(MLR)。於培养中不同
Normal pregnancy, although there is antigenic difference between the fetus and the mother, but not exclusive. Which the role of decidual tissue seems to have special significance. So far there is little report on decidual tissue, may be rare specimens. The authors prepared cell suspensions from decidual tissue, examined cell properties, and explored their possible immunological effects during the first trimester of pregnancy. 5-12 weeks pregnant women from gestational age abortion in the treatment of decidual tissue curettage. Remove villi and other tissues, centrifuged by the cell separation fluid: the interface cells were taken and the cell viability and morphology were examined by staining. Mixed lymphocyte reaction (MLR) is performed with normal volunteers or with maternal blood mononuclear cells (MNCs) as responding or stimulating cells. Different in training