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近几年来研究发现神经鞘脂类,尤其是它们的一些代谢产物如鞘氨醇-1-磷酸盐(SPP)、鞘氨醇磷酸胆碱(SPPC)在调节细胞活动中起着重要的作用。SPPC为神经鞘磷脂N端脱酰基后的衍生物,其作用途径似与SPP等有差异,为此作者选用了对SPP无反应的人白血病HL-60细胞及人嗜中性白细胞,并以此研究SPPC与SPP等神经鞘脂类是否作用于不同的G蛋白偶联受体。 在培养的HL-60细胞中导入双丁酰环磷腺苷
In recent years, studies have found that sphingolipids, especially some of their metabolites such as sphingosine-1-phosphate (SPP) and sphingosinephosphate (SPPC) play an important role in the regulation of cell activity. SPPC is a derivative of N-terminal deacylated sphingomyelin, and its action pathway seems to be different from that of SPP. Therefore, the authors chose human leukemia HL-60 cells and human neutrophils that did not respond to SPP, To investigate whether sphingolipids such as SPPC and SPP act on different G protein-coupled receptors. BifunctionylcAMP was introduced into cultured HL-60 cells