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目的建立HPLC法同时测定蒙古黄芪药材中的毛蕊异黄酮苷和芒柄花素量的方法,考察不同产地、加工方法、栽培年限对蒙古黄芪质量的影响。方法色谱柱为Dikma C18(250 mm×4.6 mm,5μm);流动相为乙腈-0.2%甲酸水溶液梯度洗脱;体积流量1.0 m L/min;检测波长260 nm;柱温30℃。结果通过对蒙古地区11个不同产地的40批次蒙古黄芪药材进行分析,比较毛蕊异黄酮苷和芒柄花素量得出,蒙古黄芪的产地加工方式最佳为清洗后自然晾晒干燥;巴彦淖尔市乌拉特中旗明安镇头份子地区为蒙古黄芪最佳栽培种植基地;生长年限二年、三年的蒙古黄芪栽培品种质量差异不大;蒙古黄芪野生品种质量优于栽培品种。结论该方法简单快捷,适合于黄芪中异黄酮类化合物的定量测定研究,为今后蒙古黄芪药材的栽培种植和加工方式合理标准的制定提供科学、可靠的依据。
OBJECTIVE To establish a HPLC method for simultaneous determination of calyxiflorin and forsythia on Mongolian Astragalus membranaceus, and investigate the effects of different producing areas, processing methods and cultivation years on the quality of Astragalus membranaceus in Mongolia. Methods The chromatographic column was Dikma C18 (250 mm × 4.6 mm, 5 μm). The mobile phase was acetonitrile-0.2% aqueous solution of formic acid. The volume flow rate was 1.0 mL / min. The detection wavelength was 260 nm. The column temperature was 30 ℃. Results Forty batches of Radix Astragali from different regions of Mongolia were analyzed. The results showed that the best way to produce Radix Astragali was to dry and dry naturally after washing. Bayannaoir The city of Wulatezhong Banner Mingan Town, the first area for the Mongolian Astragalus cultivation and cultivation of the best base; growth of two years, three years of Mongolia Astragalus cultivars quality difference is not significant; Mongolian wild Astragalus quality is better than cultivars. Conclusion The method is simple and rapid, suitable for the quantitative determination of isoflavones in Radix Astragali, and provides a scientific and reliable basis for the future development of cultivation and processing methods of Radix Astragali in Mongolia.