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以D101大孔吸附树脂为吸附剂,将对小果博落回全草的酸水提取物分为50%乙醇洗脱部分和丙酮洗脱部分。对两部分洗脱物分别进行硅胶柱色谱分离,由50%乙醇洗脱部分得到5个生物碱:二氢血根碱(1),二氢白屈菜红碱(2),6-乙氧基血根碱(3),6-丙酮基血根碱(4)和Bocconarborine A(5)。由丙酮洗脱部分得到原阿片碱(6),α-别隐品碱(7),β-别隐品碱(8)和小檗红碱(9)。采用波谱分析对化合物进行了结构表征,结果表明,化合物3、4、5、9是首次从该植物中分离得到。
Using D101 macroporous adsorption resin as adsorbent, the acid-water extract of the whole plant was separated into 50% ethanol elution fraction and acetone elution fraction. The two fractions were separated by silica gel column chromatography, respectively. Five alkaloids were eluted from 50% ethanol: dihydromylline (1), dihydroquercetin (2), 6-ethoxy Primer (3), 6-acetonylsine (4) and Bocconarborine A (5). Protoporphyrins (6), α-allodipine (7), β-allodipine (8) and berberine (9) were obtained from the eluted fractions of acetone. The structures of the compounds were characterized by spectral analysis. The results showed that compounds 3, 4, 5 and 9 were isolated from this plant for the first time.