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本文对如何提高籼粳杂交花培的愈伤组织诱导率和提高分化绿苗的成苗率等方面进行了探讨。研究结果表明,接种前以8～10℃的低温对幼穗处理4～8天,愈伤组织诱导率可提高80%～100%;继代转接2～3次,能显著地扩大愈伤组织量;采用绿色芽点分取转接和分化绿苗营养液水培法,壮苗壮根,提高成苗率达70%。
In this paper, we discussed how to improve the callus induction rate and the seedling rate of differentiated green seedlings of hybrid indica and japonica hybrids. The results showed that the callus induction rate can be improved by 80% -100% for the young ear treated with low temperature of 8 ~ 10 ℃ for 4 ~ 8 days before inoculation; The amount of tissue; using green bud points transfer and differentiation of green seedlings nutrient solution hydroponics, strong seedlings and strong roots, raising seedling rate of 70%.