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目的研究大鼠局灶性脑缺血/再灌注后缺血脑区缓激肽含量的变化。方法用线栓法制作SD大鼠大脑中动脉阻塞的脑缺血/再灌注动物模型,用免疫细胞化学法结合图像分析技术,检测缺血脑区缓激肽样免疫反应阳性物的平均光密度(A)值作为缓激肽的相对含量,比较局灶性脑缺血3h组、假手术对照组、正常对照组和再灌注30min、2h、4h、16h组缓激肽的相对含量。结果缺血脑区缓激肽样免疫反应阳性物的A值于脑缺血3h/再灌注2h后明显增高(P<0.05),随后下降至增高前水平。结论大鼠局灶性脑缺血/再灌注后,缺血脑区缓激肽含量于再灌注2h后明显增高,其可能在脑缺血后脑水肿的发生中起着重要作用。
Objective To study the changes of bradykinin content in ischemic brain regions after focal cerebral ischemia / reperfusion in rats. Methods The cerebral ischemia / reperfusion model of middle cerebral artery occlusion in SD rats was made by the method of thread plug. The average optical density of bradykinin-like immunoreactive positive cells in ischemic brain regions was detected by immunocytochemistry combined with image analysis (A) value as the relative content of bradykinin, comparing the relative content of bradykinin in focal cerebral ischemia 3h group, sham operation control group, normal control group and reperfusion 30min, 2h, 4h, 16h group. Results The A value of bradykinin-immunoreactive positive cells in ischemic brain regions was significantly increased at 3h after cerebral ischemia / 2h after reperfusion (P <0.05), and then decreased to the pre-elevated level. Conclusions After focal cerebral ischemia / reperfusion in rats, bradykinin content in ischemic brain regions increased significantly 2 h after reperfusion, which may play an important role in the development of cerebral edema after cerebral ischemia.