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目的:探究miR-146a在口腔鳞状细胞癌(OSCC)组织及细胞中的表达及其对口腔鳞癌细胞生物学行为能力的影响.方法:采用荧光定量聚合酶链反应(RT-PCR)法检测OSCC组织及细胞中miR146a的表达,观察miR-146a对OSCC细胞增殖、侵袭、迁移等能力的影响.结果:与癌旁组织相比OSCC组织中miR-146a呈明显高表达(P＜0.05);与正常口腔上皮细胞HOK相比,OSCC细胞系Scc9、Scc25、Cal27中miR-146a表达水平明显偏高(P＜0.05).上调miR-146a表达后,Scc9及Cal27的细胞增殖能力明显增加(P＜0.05),Scc9、Scc25、Cal27的侵袭和迁移能力明显增加(P＜0.05);抑制miR-146a表达后,Scc9、Scc25、Cal27的侵袭和迁移能力明显降低(P＜0.05).结论:miR146a在OSCC和口腔癌细胞中呈明显高表达,对于促进口腔癌的增殖、侵袭和迁移具有重要意义.“,”Objective:To explore the expression and its functional role of miR-146a in oral squamous cell carcinoma (OSCC) tissues.Methods:RT-PCR was performed to detect the expression level of miR-146a in OSCC tissues and OSCC cell lines.CCK8 assay,transwell assay and wound healing assay were performed to observer cell proliferation,invasion and migration abilities.Results:The expression of miR-146a was significantly higher in OSCC tissues compared to the adjacent tissues (P＜0.05),and the expression of miR-146a in OSCC cell lines such as Scc9,Scc25,and Cal27 were substantially higher than the normal oral epithelial cells (HOK) (P＜0.05).The proliferation,invasion and migration abilities were substantially enhanced in Scc9 and Cal27 cells transfected with miR -146a mimics (P＜0.05).The invasion and migration abilities were substantially decreased in Scc9,Scc25,and Cal27 cells transfected with miR-146a inhibitor (P ＜0.05),but the proliferation abilities showed no statistically diffierents in Scc9,Scc25,and Cal27 cells transfected with miR-146a inhibitor.Conclusion:miR-146a shows a significant expression in OSCC tissues and cell lines,is correlated with proliferation,invasion and migration of OSCC.