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目的探讨癌基因。ｃ－ｅｒｂＢ－２产物、抑癌基因ｐ５３突变型蛋白的过度表达以及增殖细胞核抗原标记指数（ＰＣＮＡ－ＬＩ）与乳腺浸润性癌临床病理指标的联系及其预后价值。方法随机收集７９例临床随访资料齐备的病人，用免疫组化ＡＢＣ法检测其ｃ－ｅｒｂＢ－２、ｐ５３和ＰＣＮＡ的表达情况，用Ｃｏｘ回归分析其预后意义。结果ｃ－ｅｒｂＢ－２和ｐ５３过度表达的阳性率分别为３１．７％和４８．１％。ＰＣＡＮ－ＬＩ中位数为１７．６％。ｃ－ｅｒｂＢ－２与ｐ５３过度表达之间以及其与ＰＣＮＡ－ＬＩ、肿瘤大小、组织学分级、腋淋巴结状态等指标间均无明确的相互关联。ＰＣＮＡ－ＬＩ与组织学分级呈正相关，但高ＰＣＮＡ－ＬＩ与不良预后无明显关联。Ｃｏｘ回归分析显示，腋淋巴结、ｃ－ｅｒｂＢ－２和ｐ５３过度表达等３项指标反映不良预后，危险度分别是３．３０６、１．４１９６、１．４９８４。结论ｃ－ｅｒｂＢ－２和ｐ５３过度表达是两项危险度仅次于腋淋巴结状态的反映乳腺浸润性癌不良预后的指标，ＰＣＮＡ－ＬＩ无预后价值。
Objective To investigate oncogenes. The relationship between c-erbB-2 product, tumor suppressor gene p53 mutant protein overexpression and proliferating cell nuclear antigen marker index (PCNA-LI) and clinicopathological parameters of breast invasive carcinoma and its prognostic value. Methods 79 patients with complete clinical follow-up data were randomly collected. The expression of c-erbB-2, p53, and PCNA was detected by immunohistochemical ABC method. The prognostic significance was analyzed by Cox regression analysis. Results The positive rates of c-erbB-2 and p53 overexpression were 31.7% and 48.1%, respectively. The median PCAN-LI was 17.6%. There was no clear correlation between c-erbB-2 and p53 overexpression and its association with PCNA-LI, tumor size, histological grade, and axillary lymph node status. PCNA-LI was positively correlated with histological grade, but high PCNA-LI was not significantly associated with poor prognosis. Cox regression analysis showed that three indicators such as axillary lymph node, c-erbB-2 and p53 overexpression, reflected poor prognosis, the risk was 3.306, 1.4196, 1.4984, respectively. Conclusion The overexpression of c-erbB-2 and p53 is an indicator of poor prognosis of breast invasive carcinoma after the risk of both axillary lymph nodes. PCNA-LI has no prognostic value.