论文部分内容阅读
目的:探讨不同脑温状态下银杏叶提取物(GBE)对大鼠脑缺血再灌注损伤的影响。方法:将大鼠随机分为常温假手术组、常温脑缺血对照组、亚低温脑缺血组、轻度高温银杏叶组、常温银杏叶组、亚低温银杏叶组,测定各组大鼠缺血脑组织SOD、GSH-Px、MDA含量,采用光镜观察各组大鼠缺血脑组织病理改变特点。结果:亚低温银杏叶组、亚低温脑缺血组、常温银杏叶组、常温脑缺血对照组、轻度高温银杏叶组大鼠缺血脑组织病理损伤呈依次加重趋势,与SOD、GSH-Px、MDA水平失衡有关。结论:①GBE对脑缺血的保护作用受不同脑温影响,37～37.5℃常温状态下GBE的神经保护作用有限,与脑温较高时GBE对氧自由基的清除能力有一定限度有关,而32～32.5℃亚低温状态下GBE对脑缺血的保护作用增强,与亚低温状态下GBE对氧自由基的清除能力增强有关。②亚低温和GBE联合干预,增强清除氧自由基的能力及增强对脑缺血的保护,可能不是单一因素的作用,而是亚低温作用为主,两种干预因素共同协同作用的结果。
Objective: To investigate the effect of ginkgo biloba extract (GBE) on cerebral ischemia-reperfusion injury in rats under different brain temperature. Methods: The rats were randomly divided into normal temperature sham operation group, normal temperature cerebral ischemia control group, mild hypothermia cerebral ischemia group, mild high temperature ginkgo biloba group, ginkgo biloba group and hypothermia ginkgo biloba group. The contents of SOD, GSH-Px and MDA in ischemic brain tissue were observed. The pathological changes of ischemic brain tissue were observed by light microscope. Results: The pathological changes of ischemic brain tissue in mild hypothermia Ginkgo biloba group, mild hypothermia cerebral ischemia group, normal temperature Ginkgo biloba group, normal temperature cerebral ischemic control group and mild high temperature Ginkgo biloba group tended to aggravate gradually. Compared with SOD, GSH -Px, MDA level imbalances. CONCLUSION: ① The protective effect of GBE on cerebral ischemia is influenced by different brain temperatures. The protective effect of GBE at 37 ~ 37.5 ℃ is limited, which is related to the limited ability of GBE to scavenge oxygen free radicals The protective effects of GBE on cerebral ischemia at 32 ~ 32.5 ℃ in mild hypothermia were enhanced, which were related to the enhanced ability of GBE to scavenge oxygen free radicals under mild hypothermia. ② The combination of mild hypothermia and GBE may enhance the ability to scavenge oxygen free radicals and enhance the protection of cerebral ischemia, which may not be a single factor but a result of hypothermia and synergistic effect of two interventions.