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为调控骨组织工程支架的力学性能和降解性能,采用相分离方法,以冰醋酸-水为共溶剂配制聚合物溶液,以NaOH溶液为凝固剂,以CS为添加剂制备壳聚糖(CS)/聚己内酯(PCL)-聚乳酸(PLA)三维多孔支架,研究了聚合物质量比对支架结构、形貌、孔隙率、力学性能和降解性能的影响。实验结果表明,CS和基体存在相互作用,CS有利于形成三维相互贯通的微孔结构,但CS的存在会使基体中各组分的熔点降低。随着PCL和PLA用量比例的改变,孔径范围和微孔形貌发生了一系列的变化。当PCL∶PLA为2∶4和3∶3时,所制备的支架孔隙率均大于90%,当进一步增大PCL质量比时,孔隙率迅速下降。抗压测试表明,所制备的支架弹性模量为0.8～8.0 MPa。降解性能分析表明,4周以后,当PCL∶PLA为3∶3时,质量损失率最大,达到5.94%。该分析表明采用相分离法,通过调节PCL和PLA的质量比可制备形貌、孔隙率、降解速率和力学性能满足要求的三维多孔支架材料,有望应用在软骨组织工程上。
In order to control the mechanical properties and degradation performance of the scaffolds for bone tissue engineering, the phase separation method was used to prepare the polymer solution with glacial acetic acid-water as co-solvent, NaOH solution as coagulant and CS as additive to prepare chitosan / Polycaprolactone (PCL) -polylactic acid (PLA) three-dimensional porous scaffolds were used to investigate the effect of polymer mass ratio on the structure, morphology, porosity, mechanical properties and degradation properties of scaffolds. The experimental results show that there is interaction between CS and the matrix, and CS is conducive to the formation of three-dimensional microporous structure. However, the melting point of each component in the matrix decreases due to the presence of CS. With the change of the proportion of PCL and PLA, a series of changes have taken place in the pore size range and micropore morphology. When PCL: PLA was 2: 4 and 3: 3, the scaffold porosity was greater than 90%. When the PCL mass ratio was further increased, the porosity decreased rapidly. The compression tests showed that the elastic modulus of the stent prepared was 0.8 ~ 8.0 MPa. Degradation analysis showed that after 4 weeks, when PCL: PLA was 3: 3, the mass loss rate was the largest, reaching 5.94%. The analysis shows that the phase separation method can be applied to the cartilage tissue engineering by adjusting the mass ratio of PCL and PLA to prepare the three-dimensional porous scaffold material with topography, porosity, degradation rate and mechanical properties.