谷氨酸单钠致大鼠肾上腺组织化学变化的研究

• 作 者: 王秀琴 曾晓蓓 米娜娃 杨荪 王蓬文
• 机 构: 首都医科大学组织胚胎学教研室,首都医科大学病理解剖学教研室
• 出 处: 首都医科大学学报
• 发表日期: 1998年02期
• 关键词: 大鼠 肾上腺组织 酶组织化学 非特异性酯酶 琥珀酸脱氢酶 脂类 三磷酸腺苷酶 磷酸脱氢酶 乳酸脱氢酶 显微分光光度计

观察谷氨酸单钠（ＭＳＧ）对大鼠肾上腺酶和脂类的影响。动物分为新生期（１１ｄ）、青春期（５２ｄ）、成年期（１００ｄ）３组。用酶组织化学方法显示琥珀酸脱氢酶（ＳＤＨ）、乳酸脱氢酶（ＬＤＨ）、３β羟甾脱氢酶（３βＨＳＤＨ）、葡萄糖６磷酸酶（Ｇ６Ｐａｓｅ）、葡萄糖６磷酸脱氢酶（Ｇ６ＰＤＨ）、非特异性酯酶（ＮｓＥ）、三磷酸腺苷酶（ＡＴＰａｓｅ），用油红Ｏ法显示脂类。用半定量和显微分光光度计进行定量测定。结果：１１ｄ，ＭＳＧ组上述指标高于对照组；而５２ｄ和１００ｄ，ＭＳＧ组的ＬＤＨ高于对照组，其他指标都低于对照组

To observe the effects of monosodium glutamate (MSG) on adrenal enzymes and lipids in rats. The animals were divided into three groups: neonatal (11d), adolescent (52d), adult (100d). The enzyme histochemical method showed that succinate dehydrogenase (SDH), lactate dehydrogenase (LDH), 3  βhydroxy steroid dehydrogenase (3  β  HSDH), glucose  6  phosphatase (G  6 Pase), glucose  6phosphate dehydrogenase (G6PDH), nonspecific esterase (NsE), ATPase (ATPase), with oil red O method shows lipids. Quantitative determination with semi-quantitative and micro-spectrophotometer. Results: On the 11th day, the above indexes in MSG group were higher than those in control group; while the LDH levels in MSG group on 52d and 100d were lower than those in control group, while those in other groups were lower than those in control group