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[目的]建立同时检测土壤中多菌灵、菌核净、速克灵、咪鲜胺4种杀菌剂的方法。[方法]利用紫外分光光度计扫描4种杀菌剂溶液的紫外吸收值,以确定最佳吸收波长,再通过高效液相色谱(HPLC)法同时对4种杀菌剂做定性和定量检测时所需的流动相组成、流速等色谱条件,以及土壤检测时所需提取液进行筛选。[结果]4种杀菌剂在紫外210 nm条件下均有很高的吸收值;当流动相为乙腈-水(体积比65:35)、流量为1.0 m L/min或0.75 m L/min,以及流动相为乙腈-水(体积比60:40)、流量为0.75 m L/min时,4种杀菌剂物质峰的分离度均大于1.5,可以完全分离。用乙腈作为提取液时进行回收率的检测,4种杀菌剂的回收率在98.44%~102.39%之间,且提取液中杂质较少,对杀菌剂检测的干扰较小。[结论]所建立检测方法,可用于多菌灵、菌核净、速克灵、咪鲜胺1种或几种杀菌剂的土壤残留检测,方法简单可行。
[Objective] The research aimed to establish a method for the simultaneous detection of four fungicides such as carbendazim, sclerotinum, saccharin and prochloraz in soil. [Method] The UV absorption of 4 bactericide solutions was scored by UV spectrophotometer to determine the optimal absorption wavelength. Then the four bactericides needed for qualitative and quantitative detection simultaneously by high performance liquid chromatography (HPLC) Of the mobile phase composition, flow rate and other chromatographic conditions, as well as the soil extract required for screening. [Result] The four kinds of bactericides had high absorbance under UV 210 nm. When the mobile phase was acetonitrile - water (volume ratio 65:35), the flow rate was 1.0 m L / min or 0.75 m L / min, When the mobile phase was acetonitrile-water (60:40 by volume), the separation of the four fungicide peaks was more than 1.5 at a flow rate of 0.75 m L / min, which could be completely separated. When acetonitrile was used as the extract, the recovery rate of the four fungicides was between 98.44% and 102.39%, and the impurities in the extract were less, which had less interference to the fungicide detection. [Conclusion] The established detection method could be applied to the determination of soil residues of carbendazim, sclerotinum, scapolin, prochloraz or several bactericides. The method was simple and feasible.