论文部分内容阅读
目的：探讨不明原因早期自然流产患者蜕膜中淋巴细胞表面CD3、CD4、CD8分化抗原簇的表达。方法：以10例正常妊娠早期人工流产的健康孕妇作为对照组，采用间接免疫荧光标记及流式细胞计数的方法，对10例患者蜕膜组织中的淋巴细胞表面三种分化抗原簇的表达进行了检测。结果：实验组与对照组相比较，蜕膜淋巴细胞表面CD3、CD4、CD8的表达未见显著性改变（P均＞0．05），但CD4+／CD8+比值显著升高（P＜0．005），分别为1．81±0．07、1．71±0．13。结论：不明原因早期自然流产患者蜕膜淋巴细胞表面CD4+／CD8+比值发生了改变，这或许可作为判断妊娠能否正常进行的一项重要指标．并为研究此种疾病的免疫学发病因素提供了一定的实验室依据。
Objective: To investigate the expression of CD3, CD4 and CD8 differentiated antigen clusters on the surface of lymphocytes in decidua of patients with unexplained early spontaneous abortion. Methods: Ten pregnant women with normal pregnancy induced abortion were used as control group. Indirect immunofluorescence labeling and flow cytometry were used to detect the expression of three differentiated antigen clusters on the surface of lymphocytes in decidua of 10 patients The test. Results: Compared with the control group, the expression of CD3, CD4 and CD8 on the decidual lymphocytes showed no significant change (P> 0.05), but the ratio of CD4 + / CD8 + was significantly increased ), Respectively, 1.81 ± 0.07,1.71 ± 0.13. Conclusion: The CD4 + / CD8 + ratio of decidual lymphocytes in patients with spontaneous abortion of unknown causes has been changed, which may be used as an important index to judge the normal pregnancy. And provide some laboratory evidence for studying the immunological factors of this disease.