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目的 研究碱性成纤维细胞生长因子 (basicfibroblastgrowthfactor,bFGF)在人眼中的表达及对白内障患者人晶体上皮细胞 (humanlensepithelialcells ,HLEC)增殖的影响。方法 取人眼球免疫组化测定bFGF蛋白 ;白内障术中取晶体前囊膜bFGF培养 ,4 8小时后免疫组化染色和JDIAS医用多功能图象分析软件测定增殖细胞核抗原 (proliferatingcellnuclearantigen ,PCNA)阳性面积率。结果 bFGF在晶体及晶体周围组织广泛存在 ;其促白内障患者HLEC增殖作用呈剂量依赖性 ,且与白内障患者年龄呈负相关 (P <0 0 5 )。结论 bFGF促白内障患者术后残留HLEC增殖 ,参与了后发障的形成。
Objective To study the expression of basic fibroblast growth factor (bFGF) in human eyes and its effect on the proliferation of human lens epithelial cells (HLEC) in cataract patients. Methods Human bFGF protein was determined by eyeball immunohistochemistry. The cFGF was incubated in the anterior lens capsule of bFGF after 48 hours. The positive area of proliferating cell nuclear antigen (PCNA) was measured by immunohistochemistry and JDIAS medical image analysis software 48 h after cataract surgery. rate. Results The bFGF was widely distributed around the crystal and the lens. The proliferative effect of bFGF in cataract patients was dose-dependent, and was negatively correlated with the age of cataract patients (P <0.05). Conclusions bFGF promotes the proliferation of residual HLEC after cataract surgery and is involved in the formation of posterior obstacle.