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Objective:To investigate the in vitro antioxidant and antimicrobial activities along with phytochemical screening of organic and aqueous extracts of leaf and stem of Suaeda maritima (Dumort).a mangrove associate from Bhitarkanika of Odisha.India.Methods:Antioxidant activity of the crode extracts was evaluated in terms of total antioxidant capacity,total phenol content,ascorbic acid content,DPPH radical scavenging,metal chelating,nitric oxide scavenging,and reducing power etc.The antimicrobial activity of the plant was determined by agar well diffusion method along with MIC and BBC carried out by microdilulion techniques against 10 gram positive and gram negative human pathogenic bacteria.The qualitative and qualitative phytochemical screening were carried out by standard biochemical assays.Results: Out of the seven antioxidant bioassavs,both the leaf and stem extracts were found to posses strong antioxidant properties of 70%to 92%for phenol,total antioxidant capacity,DPPH free radical scavenging aclivitv and fairly good ascorbic acid content,metal chelating(1.33%-22.55%). reducing power(0.01-0.12) and nitric oxide scavenging(0.84%-66.99%) activities.Out of the four extracts evaluated for antimicrobial activity,two leaf extracts such as acetone and ethanol showed promising activity against four pathogenic bacteria and one stem methanol extracts against one pathogenic bacteria when compared with amoxcycillin as standard.The MIC and MBC values of the antimicrobial extracts ranged between 2.5 to 5.0 mg/mL.Screening of phytochemicals showed presence of carbohydrates,protein,tannins,alkaloids and flavonoids in comparatively higher amount than other phytochemicals tested.Conclusions:The present study reveals the presence of potential antioxidants and antimicrobial properties in the plant extract which could be exploited for pharmaceutical application.
Objective: To investigate the in vitro antioxidant and antimicrobial activities along with phytochemical screening of organic and aqueous extracts of leaf and stem of Suaeda maritima (Dumort). A mangrove associate from Bhitarkanika of Odisha. India. Methods: Antioxidant activity of the crode extracts was evaluated in terms of total antioxidant capacity, total phenol content, ascorbic acid content, DPPH radical scavenging, metal chelating, nitric oxide scavenging, and reducing power etc. The antimicrobial activity of the plant was determined by agar well diffusion method along with MIC and BBC carried out by microdilulion techniques against 10 gram positive and gram negative human pathogenic bacteria. The qualitative and qualitative phytochemical screening were carried out by standard biochemical assays. Results: Out of the seven antioxidant bioassavs, both the leaf and stem extracts were found to posses strong antioxidant properties of 70% to 92% for phenol, total antioxidant capacity, DPPH free radical reducing power (0.01-0.12) and nitric oxide scavenging (0.84% -66.99%) activities. Out of the four as evaluated for antimicrobial activity, two leaf extracts such as acetone and ethanol showed promising activity against four pathogenic bacteria and one stem methanol extracts against one pathogenic bacteria when compared with amoxcycillin as standard. MIC and MBC values of the antimicrobial extracts ranged between 2.5 and 5.0 mg / mL. phytochemicals showed presence of carbohydrates, protein, tannins, alkaloids and flavonoids in comparatively higher amount than other phytochemicals tested. Conclusions: The present study reveals the presence of potential antioxidants and antimicrobial properties in the plant extract which could be exploited for pharmaceutical application.