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目的通对美国进境的环保微生物菌剂恶臭假单胞菌进行鉴定。方法从该样品中分离、纯化得到菌株,进行形态学、生理生化测定,并提取其基因组DNA,进行16SrDNA扩增、基因测序等,分析该菌是否是恶臭假单胞菌。结果综合形态学、生理生化特征,16SrDNA扩增片段序列分析及在数据库NCBI上比对结果,分析该样品中不含有恶臭假单胞菌,与申报进境的商品不符。结论首次截获从美国进境的不合格环保微生物菌剂恶臭假单胞菌。
Objective To pass the identification of Pseudomonas putida, an environmental microbial inoculum imported into the United States. Methods The strains were isolated and purified from the samples. Morphological, physiological and biochemical tests were carried out. The genomic DNA was extracted and the 16SrDNA was amplified and sequenced. The results showed that the strain was Pseudomonas putida. Results The results of morphological, physiological and biochemical characteristics, sequence analysis of 16S rDNA amplified fragment and comparison results on the NCBI database showed that this sample did not contain Pseudomonas putida, which was inconsistent with the imported products. Conclusion For the first time, Pseudomonas putida, a non-conforming environmental microbial inoculum, was imported from the United States.