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应用淀粉凝胶电泳法 ,分析了具有代表性的 4 4 0 8份中国栽培稻种质资源的 12个同工酶基因位点的等位基因酶谱 (Pgi1,Pgi2 ,Amp1,Amp2 ,Amp3,Amp4 ,Sdh1,Adh1,Est1,Est2 ,Est5和 Est9)。结果指出 ,被测的中国栽培稻含有 5 2个同工酶等位基因 ,占亚洲栽培稻已鉴定出的 5 4个等位基因的 96 .3% ,其等位基因频率变幅为 0 .0 0 1～ 0 .994 ,基因多样性指数 (H a)为 0 .0 12～ 0 .5 4 7。平均基因多样性指数 (H t) ,同工酶遗传型多样性指数 (H p)和平均多态性指数(DP)分别为 0 .2 4 8,3.84 5和 17.7%。基因频率低于 0 .0 5的等位基因共 31个 ,占 5 9.6 % ;基因频率在 0 .91～ 0 .95的等位基因 5个 (Amp1- 2 ,Amp3- 1,Adh1- 1,Est1- 1和 Est9- 2 ) ,占 9.6 ;基因频率高于 0 .95的等位基因 2个 (Amp4 - 1和Est5 - 1) ,占 3.8。研究发现 ,Pgi1- 5等位基因仅存在于中国栽培稻中。我国六大稻区中 ,以西南稻区的同工酶平均基因多样性指数为最高 (0 .2 6 6 ) ,其次为华中和华南稻区。华中稻区的同工酶平均等位基因数最高 (3.75 ) ,其次为西南稻区。研究结果证实中国栽培稻同工酶具有丰富的遗传多样性
The isozyme patterns of 12 isozyme loci (Pgi1, Pgi2, Amp1, Amp2, Amp3, Amp4, Sdh1, Adh1, Est1, Est2, Est5 and Est9). The results showed that the tested Chinese cultivated rice contained 52 isozyme alleles, accounting for 96.3% of the 5 4 alleles identified in Asian cultivated rice, and its allele frequency was changed to zero. 0 0 1 ~ 0. 994, and the genetic diversity index (H a) was 0.12 ~ 0.54 7. The average genetic diversity index (H t), isozyme genetic diversity index (H p) and average polymorphism index (DP) were 0.248, 3.8445 and 17.7% respectively. There were 31 alleles with a frequency of less than 0.050, accounting for 59.6%. Five alleles (Amp1-2, Amp3-1, Adh1-1, Est1-1 and Est9-2, accounting for 9.6%. Two alleles (Amp4 - 1 and Est5 - 1) with a frequency of more than 0.95 accounted for 3.8%. The study found that the Pgi1-5 allele exists only in Chinese cultivated rice. Among the six rice regions in China, the average isozyme isozyme index in the southwest rice region was the highest (0.266), followed by the rice regions in central and southern China. The average number of isozymes in central China rice area was the highest (3.75), followed by the southwest rice area. The results confirmed that Chinese cultivated rice isozymes have rich genetic diversity