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目的探讨弥漫性大B细胞淋巴瘤(DLBCL)中遍在蛋白活化酶E1(UBE1)和凋亡抑制基因Bcl-2蛋白的表达及临床病理意义。方法收集80例DLBCL组织和30例良性淋巴组织反应性增生(RH),应用免疫组化技术(EnVision二步法)对DLBCL组织进行免疫分型并检测其免疫表型GCB型、ABC型中UBE1、Bcl-2蛋白的表达;同时检测UBE1、Bcl-2蛋白在RH组织中的表达。分析UBE1、Bcl-2蛋白表达与DLBCL患者临床病理特征的关系,及DLBCL组织中UBE1与Bcl-2表达的相关性。结果 DLBCL组织中UBE1阳性表达率为65.0%(52/80),RH组织中阳性表达率为13.3%(4/30),两者差异具有统计学意义(P<0.05);GCB型和ABC型的阳性表达率分别为53.3%(16/30)、72.0%(36/50),两者差异具有统计学意义(P<0.05)。DLBCL组织中Bcl-2阳性表达率为51.3%(41/80),RH组织中阳性表达率为10.0%(3/30),两者差异具有统计学意义(P<0.05);GCB型和ABC型的阳性表达率分别为36.7%(11/30)、60.0%(30/50),两者差异具有统计学意义(P<0.05)。在DLBCL组织中,UBE1的阳性表达率与患者的年龄、累及结外数目有关(P<0.05);Bcl-2的阳性表达率与DLBCL患者的年龄、Ann Arbor分期、累及结外数目、国际预后指数等病理特征有关(P<0.05)。UBE1在ABC型中的表达与Bcl-2的表达呈正相关(r=0.582,P=0.001)。结论 UBE1、Bcl-2蛋白在DLBCL的ABC型中表达较高,在肿瘤的发生、发展及侵袭中起着一定的作用,UBE1与Bcl-2蛋白的高表达可能提示DLBCL患者的不良预后。
Objective To investigate the expression of UBE1 and Bcl-2 in diffuse large B cell lymphoma (DLBCL) and its clinicopathological significance. Methods 80 cases of DLBCL tissues and 30 cases of benign lymphoid tissue reactive hyperplasia (RH) were collected. Immunohistochemistry (EnVision two-step method) was used to immunophenotype the DLBCL tissues and detect the immunophenotype GCB, ABC type UBE1 , Bcl-2 protein expression; simultaneous detection of UBE1, Bcl-2 protein expression in RH tissue. The relationship between the expression of UBE1 and Bcl-2 protein and the clinicopathological features of DLBCL and the relationship between UBE1 and Bcl-2 in DLBCL were analyzed. Results The positive expression rate of UBE1 in DLBCL tissues was 65.0% (52/80), while the positive expression rate in RH tissues was 13.3% (4/30). There was a significant difference between the two groups (P <0.05) The positive rates were 53.3% (16/30) and 72.0% (36/50), respectively, with statistical significance (P <0.05). The positive rate of Bcl-2 in DLBCL was 51.3% (41/80), while the positive rate in RH was 10.0% (3/30), the difference was statistically significant (P <0.05) The positive expression rates were 36.7% (11/30) and 60.0% (30/50), respectively, with significant difference (P <0.05). In DLBCL, the positive expression rate of UBE1 was related to the age of patients and the number of extranodal involvement (P <0.05). The positive expression rate of Bcl-2 correlated with age, Ann Arbor stage, number of extranodal involvement, international prognosis Index and other pathological features (P <0.05). The expression of UBE1 in ABC type was positively correlated with the expression of Bcl-2 (r = 0.582, P = 0.001). Conclusions UBE1 and Bcl-2 proteins are highly expressed in ABC type of DLBCL and play a role in tumorigenesis, development and invasion. High expression of UBE1 and Bcl-2 protein may indicate poor prognosis in patients with DLBCL.