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前言在康乃馨脱毒种苗工厂化生产的环节中,脱毒种苗从试管苗繁殖转入生根阶段以后,诱导生根的技术便成为解决种苗供应的一个关键。显然,这也是康乃馨开发研宄中需解决的课题。以固体培养基对试管苗的发根作用而言,则试管苗往往容易在插入培养基的下部茎段和贴近培养基表面的叶片上产生愈伤组织,尔后在其上直接生根,致使这一类根系的维管束与主茎维管束不连通,生根苗移栽后很难成活。再者,用固体培养基的发根时间较长,少则2周左右,多则3～4周才开始发根,且发根株百分率很低(从试管苗茎基部发根的植株)、发根极不整齐,难以适应批量生产的要求。为此我们开始了一系列的发根方法的试验研究。从初步取得的结
Preface In the process of industrialized production of carnation virus-free seedlings, the technology of inducing rooting after removing virus-resistant seedlings from in vitro plantlet breeding to rooting stage has become a key to solve the seedling supply. Obviously, this is also a problem to be solved in the research and development of carnation. In the case of rooting of test-tube seedlings by solid medium, the test-tube plantlets tend to easily produce callus on the lower stems inserted into the medium and on the leaves adjacent to the surface of the medium and then rooted directly on them Roots of the vascular bundles and the main stem vascular bundle is not connected, it is difficult to survive after transplanting. In addition, the rooting time with solid medium is longer, ranging from about 2 weeks to as many as 3 to 4 weeks, and the percentage of hairy roots is very low (from roots of test-tube plantlets) The root is very irregular, it is difficult to adapt to the requirements of mass production. To this end we started a series of experimental studies of hair root method. From the initial acquisition of the knot