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目的:观察通过劳倦加饮食不节(高糖高脂膳食)复合腹腔注射链脲佐菌素造模方法能否成功复制出脾气虚证2型糖尿病动物模型。方法:健康雄性Wistar大鼠60只,体重(220±20)g,按体重随机分成二组:空白对照组(n=20)和模型组(n=40)。模型组单日喂食甘蓝15~20 g/只,自由饮水,游泳至耐力极限;双日高糖高脂膳食持续喂养,共计12周,第4周后给予链脲佐菌素腹腔注射。结果:模型组大鼠造模开始后,出现了游泳耐力逐日下降,食少懒动,大便溏薄,精神萎靡不振,体毛光亮度减退,从第6周后体重就开始显著增加,链脲佐菌素腹腔注射2周后空腹血糖、餐后2h血糖增高,甘油三酯和总胆固醇分别从第4周后表现出异常,造模结束后模型组出现高血糖症、高胰岛素血症、胰岛素抵抗,符合本研究拟建立的脾气虚证、2型糖尿病的评估标准。结论:通过劳倦加饮食不节(高糖高脂膳食)造模方法可成功复制出脾气虚证2型糖尿病动物模型。
OBJECTIVE: To observe whether animal models of type 2 diabetes with spleen-qi deficiency syndrome can be successfully replicated through intraperitoneal injection of streptozotocin (STZ) by labor plus dietary improper diet (high glucose and high fat diet). Methods: Sixty healthy male Wistar rats weighing 220 ± 20 g were randomly divided into two groups: control group (n = 20) and model group (n = 40). The model group fed 15 ~ 20 g / day of cabbage on a single day, drinking water freely and swimming to the endurance limit. The double-day high-sugar and high-fat diet was fed continuously for 12 weeks and the fourth week later, streptozotocin was injected intraperitoneally. Results: After the beginning of modeling, the endurance of swimming in rats in the model group decreased day by day, the food was lazy, the stool was thin, the spirit was depressed and the body brightness decreased. The body weight began to increase significantly after 6 weeks. After two weeks of intraperitoneal injection of mycotoxin, fasting blood glucose and 2h postprandial hyperglycemia were increased. Triglyceride and total cholesterol were abnormal after 4 weeks. After the model was established, hyperglycemia, hyperinsulinemia and insulin resistance , In line with the study to be established spleen deficiency syndrome, type 2 diabetes evaluation criteria. Conclusion: The animal model of type 2 diabetes with spleen-qi deficiency syndrome can be successfully replicated through the model of laboriousness plus diet-free (high-sugar and high-fat diet) modeling.