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烟叶钾含量是评定烤烟品质的重要指标之一,它与烟叶的燃烧性、降低危害物质、增强烟叶的香吃味等密切相关。本试验以钾元素富集能力较强的烟草为材料,采用不同的钾离子浓度处理烟苗,研究不同钾离子浓度对DNA甲基化变化的影响,试验结果表明,0、15 mmol/L、30 mmol/L、45 mmol/L和60 mmol/L硝酸钾浓度处理烟苗的总甲基化比例为33.26%、32.82%、29.20%、28.94%和27.43%,全甲基化比率为22.45%、22.17%、19.47%、19.43%和17.71%。随着钾离子处理浓度的增高,甲基化条带比率、全甲基化条带百分比均逐渐降低,DNA甲基化的变化可能是烤烟幼苗调控基因表达响应钾胁迫的方式之一。
The potassium content of tobacco leaves is one of the important indexes to evaluate the quality of flue-cured tobacco. It is closely related to the combustibility of tobacco leaves, reducing the harmful substances and enhancing the fragrant taste of tobacco leaves. In this experiment, tobacco with stronger potassium enrichment ability was used as material, and different concentrations of potassium ions were used to treat tobacco seedlings to study the effects of different concentrations of potassium ions on DNA methylation. The results showed that 0,15 mmol / L, The total methylation rates of tobacco seedlings treated with 30 mmol / L, 45 mmol / L and 60 mmol / L potassium nitrate were 33.26%, 32.82%, 29.20%, 28.94% and 27.43%, respectively. The full methylation ratio was 22.45% , 22.17%, 19.47%, 19.43% and 17.71% respectively. With the increase of potassium concentration, the percentage of methylated bands and full methylated bands decreased gradually. The changes of DNA methylation may be one of the ways that tobacco plants regulate the gene expression in response to potassium stress.