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为进一步了解孤啡肽在脊髓水平是否具有抗伤害及抗炎作用,本实验在具有多种痛行为表现的蜜蜂毒模型上观察了鞘内注射孤啡肽对大鼠一侧后足底注入蜜蜂毒所诱致的同侧自发缩足反射、原发热和机械性痛敏以及注射部位炎症反应的影响,同时观察了新的高选择性孤啡肽受体拮抗剂CompB的作用。结果表明:与生理盐水对照组比较,鞘内注射孤啡肽(3、10、30nmol/10μl)对蜜蜂毒诱发的自发缩足反射次数的抑制作用随剂量提高而增大,抑制率分别为37±7,43±6 and 57±11%(三个剂量vs对照,P<0.05);而对蜜蜂毒诱发的注射部位炎症反应(爪体积、爪背腹厚度和蛋白渗出的增加)无显著影响。CompB(30nmol)可完全翻转10nmol孤啡肽对自发缩足反射的抑制作用。鞘内单次或重复注射孤啡肽(10nmol/10μl)对蜜蜂毒诱致的原发性热和机械性痛敏的发生和维持均无作用。本实验结果提示,外源性孤啡肽在脊髓通过孤啡肽受体的介导产生一定的镇痛作用,但是它可能仅对持续性自发痛有抑制作用,而对热和机械性痛敏及炎症反应均无影响。
In order to further understand whether orphanin has anti-injury and anti-inflammatory effects at the spinal cord level, we observed whether intrathecal injection of orphaninol into bees injected into the rat’s posterior plantar in a bee poison model with multiple pain behaviors Toxic-induced ipsilateral auto-contractive reflex, primary fever and mechanical hyperalgesia as well as the inflammatory response at the injection site, and at the same time observed the role of the new highly selective orphaninol receptor antagonist CompB. The results showed that the inhibitory effect of intrathecal orphanin (3, 10, 30 nmol / 10μl) on the frequency of spontaneous reflex reflex induced by bee venom increased with the increase of dosage, and the inhibitory rates were 37 ± 7,43 ± 6 and 57 ± 11% (three doses vs. control, P <0.05); whereas the inflammatory response at injection site (paw volume, dorsoventral thickness and protein excretion) was not significant influences. CompB (30 nmol) completely reverses the inhibitory effect of 10 nmol orphaninol on auto-contracting reflex. Intrathecal single or repeated injections of orphanin (10 nmol / 10 μl) had no effect on the onset and maintenance of primary thermal and mechanical hyperalgesia induced by bee venom. The results suggest that exogenous orphanin peptide in the spinal cord through the orphan peptide receptor-mediated produce some analgesic effect, but it may only inhibit the sustained spontaneous pain, and thermal and mechanical pain sensitivity And inflammatory reaction have no effect.