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为了阐明1999年从转基因后代遗传群体中发现的1株雄性不育植株不育基因的遗传规律及其与现有不育基因的等位性,采用表型观察测量。以及经典的自交和测交手段,研究了该不育材料败育性状的遗传规律。花器官形态特征调查表明:不育株花柱长和花柱外露长度均明显高于同质系的正常可育株,而每朵花的子房直径及花药数量没有明显差异。遗传分析表明:杂合体可育株自交,后代不育株与可育株呈1:3分离,不育株与杂合姊妹可育株测交,不育株与可育株呈1:1分离,表明该核不育材料受隐性单位点控制;与阆A(msc1)、洞A(msc3)等育性位点杂合可育株分别杂交,其F1代单株育性均得到恢复。由其F1代产生的F1:2家系中均出现不育株与可育株呈1:3和7:9两个育性分离群体,表明该材料败育基因为不同于阆A、洞A的不育基因位点。
In order to elucidate the inheritance of the sterility gene of a male sterile plant found in the genetic population of transgenic progeny in 1999 and its allelic relationship with the existing sterile gene, phenotypic observation was used to measure the genotype. As well as the classic selfing and cross dating methods, the genetic rules of abortion characters in sterile materials were studied. Morphological characteristics of floral organs were investigated. The results showed that the length of the style and the length of style were significantly higher than that of the normal fertile line. However, there was no significant difference in ovary diameter and number of anther per flower. The results of genetic analysis showed that the heterozygous fertile plants were selfed, the offspring sterile plants and fertile plants were separated 1: 3, sterile plants and hybrid sister fertile plants were detected, the sterile plants and fertile plants were 1: 1 The results showed that the nuclear sterile material was controlled by recessive single locus. The hybrid fertile plants with fertile loci such as A msc1 and msc3 were respectively crossed, . F1: 2 lines from F1 generation showed sterile and fertile plants showed 1: 3 and 7: 9 two fertility segregation groups, indicating that the material abortion gene is different from the A, A hole Sterile loci.