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目的了解超排卵周期人血清及卵泡液中胰岛素样生长因子II(IGF-II)水平,探讨IGF-II在生殖内分泌中的作用。方法采用放射免疫法(RIA)测定45例超排卵周期取卵日人血清及卵泡液中IGF-II、雌二醇(E2)、孕酮(P)、睾酮(T)、卵泡刺激素(FSH)、黄体生成素(LH)水平,分析IGF-II与卵泡发育、生殖激素及为超排卵而使用r-FSH、人绝经期促性腺激素(hMG)的剂量之间的相关性及意义。结果卵泡液IGF-II(0.60±0.69)μgL,低于血清IGF-II(1.09±1.21)μgL(z=1.975,P<0.05);卵泡液中IGF-II与E2、P水平呈正相关(r=0.620、0.509,均P<0.01);与TE2比值呈负相关(r=0.514,P<0.01),与FSH、LH水平及LHFSH比值无相关性(r=0.033、0.044、0.062,均P>0.05);与直径14mm以上的卵泡数及获卵数呈正相关(r=0.393、0.324,均P<0.05)。血清IGF-II与E2、P、T、FSH、LH水平及TE2均无相关性(均P>0.05),与直径14mm以上的卵泡数及获卵数亦无相关性(r=0.099、0.185,均P>0.05)。血清及卵泡液IGF-II水平与超排卵而使用的r-FSH、hMG剂量均无显著相关性(均P>0.05)。结论IGF-II在卵巢内调节卵巢功能中起了重要作用,可能具有促进卵泡发育、形成优势卵泡的作用,并可能促进卵泡E2、P的分泌。
Objective To investigate the level of insulin-like growth factor II (IGF-II) in human serum and follicular fluid during the superovulation cycle and to explore the role of IGF-II in reproductive endocrine. Methods Serum levels of IGF-II, estradiol (E2), progesterone (P), testosterone (T), follicle-stimulating hormone (FSH) in ovariectomized human oviduct and ovariectomized ovariectomized rats were determined by radioimmunoassay (RIA) ) And luteinizing hormone (LH) were measured. The correlation between IGF-II and follicular development, reproductive hormones and dosage of r-FSH and human menopausal gonadotropin (hMG) for superovulation was analyzed. Results The level of IGF-II in follicular fluid was significantly lower than that in serum IGF-II (1.09 ± 1.21 μg L (z = 1.975, P <0.05) (R = 0.514, P <0.01), but no correlation with FSH and LH levels and LHFSH ratio (r = 0.033,0.044,0.062, P <0.01) 0.05). There was a positive correlation between the number of follicles and the number of oocytes retrieved (P = 0.393,0.324, P <0.05). Serum IGF-II had no correlation with E2, P, T, FSH, LH and TE2 (all P> 0.05), but no correlation with the number of follicles and the number of oocytes retrieved (P> 0.05) All P> 0.05). Serum and follicular fluid IGF-II levels and superovulation r-FSH, hMG dose were not significantly correlated (P> 0.05). Conclusion IGF-II plays an important role in ovarian regulation of ovarian function, which may promote the development of follicles and form the dominant follicles, and may promote the secretion of E2 and P of follicles.