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目的 :利用大鼠小肠缺血再灌流 (IIR)模型 ,评价肿瘤坏死因子 (TNF)在IIR所致肺损伤发病过程中的作用 ;观察大黄对TNF影响 ,探讨大黄防治肠源性肺损伤的机理。方法 :SD大鼠随机分为 4组 :1)肠缺血再灌流组 (n =2 4) ;2 )假手术组 (n =16 ) ;3)大黄治疗组(n =2 4) ;4)安慰剂组 (n =16 )。以1 2 5 I标记小牛血清白蛋白 (BSA)肺摄取指数作为评价肺毛细血管通透性的指标 ,以髓过氧化物酶 (MPO)作为评价多聚核白细胞 (PMN)在组织中聚集的指标 ,采用放射免疫分析法 ,分别测定各组动物不同时间血浆及肺组织TNF含量。结果 :大黄可明显抑制再灌流导致的肺MPO活性升高 (P <0 0 1)及肠缺血期和再灌流早期出现的血浆及肺组织TNF水平升高 (P <0 0 1) ;降低肺毛细血管通透性 (P <0 0 1)。结论 :早期应用大黄有助于防止大鼠IIR所致肺损伤的发生 ,这种作用至少部分是通过抑制IIR诱导的TNF释放而实现的。
OBJECTIVE: To evaluate the role of tumor necrosis factor (TNF) in the pathogenesis of IIR-induced lung injury in rat model of intestinal ischemia-reperfusion (IIR). To observe the effect of rhubarb on TNF, and to explore the mechanism of rhubarb in preventing and treating gut-derived lung injury . Methods: SD rats were randomly divided into 4 groups: 1) intestinal ischemia-reperfusion group (n = 24); 2) sham operation group (n = 16); 3) rhubarb treatment group Placebo group (n = 16). Pulmonary peroxidase (MPO) was used as an index to evaluate pulmonary capillary permeability with 125I-labeled bovine serum albumin (BSA) as a marker of aggregation of polyneurocytopenic cells (PMN) in tissues The radioimmunoassay was used to determine the levels of TNF in plasma and lung tissue of different groups at different times. Results: Rhubarb could obviously inhibit the increase of pulmonary MPO activity induced by reperfusion (P <0.01) and the increase of TNF and TNF in plasma and lung tissue during the early stage of intestinal ischemia and reperfusion (P <0.01) Pulmonary capillary permeability (P <0.01). Conclusion: The early application of rhubarb helps to prevent the occurrence of lung injury induced by IIR in rats. This effect is achieved at least partly through the inhibition of IIR-induced TNF release.