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BACKGROUND: It has been reported that peroxisome proliferator-activated receptor γ(PPARγ) is highly expressed in lung cancer, colon cancer, and gastric cancer, as well as other tumors. OBJECTIVE: To study expression of PPARγin pituitary adenomas and analyze the role of PPARγin hormonal typing of pituitary adenomas. DESIGN, TIME AND SETTING: Semi-quantitative immunohistochemistry of pathological specimens. The experiment was conducted at the Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University between January 2002 and May 2005. MATERIALS: Surgical resection samples of pituitary adenomas from 38 cases (18 male and 20 female) were analyzed. Eight cases were determined to be invasive pituitary adenomas and 30 cases were non-invasive pituitary adenomas. Hormonal classification of the types of pituitary adenomas revealed somatotrophic adenomas in six cases, corticotrophic adenoma in five cases, prolactinomas in 13 cases, multi-hormone secreting adenomas in six cases, and eight cases of adenoma without altered endocrine function. Five autopsy specimens were collected during the same period from patients of matching age that died from unrelated diseases and were included as normal anterior pituitary controls. METHODS: Cell counts for positive immunohistochemical signals were recorded from histopathological sections. The percentage of positive cells was reported as a semi-quantitative analysis. MAIN OUTCOME MEASURES: The rate of PPARγpositive cells in different types of adenoma was based on hormonal levels and invasiveness of pituitary tumor cells. RESULTS: All tumor biopsies were determined to express PPARγ The rate of PPARγ-positive cells ranged between 8%-65% in the pituitary adenomas. According to hormonal type, PPARγexpression did not vary between the groups. In addition, there was no significant difference in PPARγexpression between the non-invasive and invasive pituitary adenomas. CONCLUSIONS: Human pituitary adenomas express PPARγ, and this expression is unrelated to hormonal type and invasiveness.
BACKGROUND: It has been reported that peroxisome proliferator-activated receptor γ (PPARγ) is highly expressed in lung cancer, colon cancer, and gastric cancer, as well as other tumors. OBJECTIVE: To study expression of PPARγin pituitary adenomas and analyze the role of The experiment was conducted at the Department of Neurosurgery, Wuxi Second Hospital Affiliated to Nanjing Medical University between January 2002 and May 2005. MATERIALS: Surgical resection Samples of pituitary adenomas from 38 cases (18 male and 20 female) were analyzed. Eight cases were determined to be invasive pituitary adenomas and 30 cases were non-invasive pituitary adenomas. Hormonal classification of the types of pituitary adenomas revealed somatotrophic adenomas in six cases , corticotrophic adenoma in five cases, prolactinomas in 13 cases, multi-hormone secreting adenom as in six cases, and eight cases of adenoma without altered endocrine function. Five autopsy specimens were collected during the same period from patients of matching age that died from unrelated diseases and were included as normal anterior pituitary controls. METHODS: Cell counts for positive immunohistochemical signals were recorded from histopathological sections. The percentage of positive cells was reported as a semi-quantitative analysis. MAIN OUTCOME MEASURES: The rate of PPARγ positive cells in different types of adenoma was based on hormonal levels and invasiveness of pituitary tumor cells. The rate of PPARγ-positive cells ranged between 8% -65% in the pituitary adenomas. According to the hormonal type, PPARγexpression did not vary between the groups. In addition, there was no significant difference in PPARγexpression between the non-invasive and invasive pituitary adenomas. CONCLUSIONS: Human pituitary adenomas expr ess PPARγ, and this expression is unrelated to hormonal type and invasiveness.