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目的 :探讨汕头地区人群HLA_DRB1等位基因分布 ,及其对风湿性关节炎 (RA)易感性及病情和预后估计的意义。方法 :用序列特异性引物基因扩增法 (PCR_SSP)对 117例RA、 38例SLE和 10 0例正常人进行DRB1等位基因鉴定 ;用序列特异性寡核苷酸探针 (SSOP)斑点杂交进行DRB1 0 4亚型鉴定。结果 :RA患者DRB1 0 4频率显著高于正常组 (4 9 6 %对 18 0 % ) ,且以DRB1 0 40 5为主要亚型 (6 2 1%对 2 7 8% )。DRB1 0 4阳性组RA较阴性组病程长、发病年龄轻 (P <0 0 5 ) ,两组间各种自身抗体阳性率无显著性差异 (P >0 0 5 )。结论 :汕头地区人群RA和DRB1 0 4显著相关 ,其主要亚型为DRB1 0 40 5。DRB1等位基因型别可作为估计RA病情及预后的指标。
Objective: To investigate HLA-DRB1 allele distribution in Shantou population and its significance for susceptibility to rheumatoid arthritis (RA) and prognosis. Methods: The DRB1 alleles were identified in 117 patients with RA, 38 with SLE and 100 with normal controls by PCR-SPECT. The sequence-specific oligonucleotide probes (SSOP) DRB1 0 4 subtype identification. Results: The frequency of DRB104 in patients with RA was significantly higher than that in controls (496% vs 180%), and DRB1 0405 was the major subtype (62.1% vs 278%). There was no significant difference in the positive rate of autoantibodies among the two groups (P> 0.05). Conclusion: There is a significant correlation between RA and DRB1 0 4 in Shantou population. The main subtype is DRB1 0 40 5. DRB1 allele can be used as an index to evaluate the condition and prognosis of RA.