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Objective To screen the phytoestrogenic effective extracts and dose of Cistanche deserticola including estrogenic and anti-estrogenic activities.Methods The effect of phytoestrogen was determined through uterus growth test in low and high estrogen female model mice.Then MTT assay of the estrogen-dependent breast cancer cells MCF-7 was conducted with the medicated serum of mice.Results After ig administration with 95%ethanol extract of C.deserticola[EECD,30 g/(kg·d)],the uterus coefficient of low estrogen model mice increased.The medicated serum of 30 g/(kg·d)EECD significantly promoted the proliferation of MCF-7 cells.40 g/(kg·d)EECD+diethylstilbestrol significantly inhibited the growth of the uterus in high estrogen model mice and the proliferation of MCF-7 cells as well.Conclusion With the dose of 30 g/(kg·d),EECD could exert quasi estrogen effect,and with the dose of 40g/(kg·d),EECD could exert the estrogen antagonistic action.The method established is accurate and reliable,which could be used for the follow-up studies on the phytoestrogen material basis of C.deserticola.
Objective To screen the phytoestrogenic effective extracts and dose of Cistanche deserticola including estrogenic and anti-estrogenic activities. Methods The effect of phytoestrogen was determined through uterus growth test in low and high estrogen female model mice. MTT assay of the estrogen-dependent breast cancer cells MCF-7 was conducted with the medicated serum of mice. Results after ig administration with 95% ethanol extract of C. deserticola [EECD, 30 g / (kgd)], the uterus coefficient of low estrogen model mice increased. medicated serum of 30 g / (kg · d) EECD significantly promoted the proliferation of MCF-7 cells. 40 g / (kg · d) EECD + diethylstilbestrol significantly inhibited the growth of the uterus in high estrogen model mice and the proliferation of MCF -7 cells as well. Confusion with the dose of 30 g / (kg · d), EECD could exert quasi estrogen effect, and with the dose of 40 g / (kg · d), EECD could exert the estrogen antagonistic action. established is accurate and reliable, whi ch could be used for the follow-up studies on the phytoestrogen material basis of C. deserticola.