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为了筛选获得最优海藻糖浓度的绵羊鲜精稀释液,试验用TRIS专利基础稀释液分别配制5个海藻糖浓度,0(A组)、5 mmol/dm~3(B组)、10 mmol/dm~3(C组)、15 mmol/dm~3(D组)、20 mmol/dm~3(E组),采用假阴道法采集5只公羊的精液,用稀释液分别对精液进行4倍稀释,置于30℃水浴杯中,稀释精液在2 h水浴杯降温到0℃后,冰水混合物保存,第0,24,72,144,216,288小时时对精子活率、精子低渗膨胀率和精子顶体完整率进行检测。结果表明:在TRIS精液稀释液中添加低于20 mmol/dm~3浓度的海藻糖不会提高被保存精液的精子活率,但可以显著提高被保存精子顶体完整率,优化的海藻糖添加剂量是5 mmol/dm~3。
In order to screen the optimal concentration of trehalose in sheep Sheep fresh dilution, the test using TRIS patent base dilution were prepared five trehalose concentrations, 0 (A group), 5 mmol / dm ~ 3 (B group), 10 mmol / The rats were randomly divided into four groups: dm ~ 3 (group C), 15 mmol / dm ~ 3 (group D) and 20 mmol / dm ~ Dilution, placed in a 30 ℃ water bath cup, dilute the sperm in 2 h water bath cup cooled to 0 ℃, the ice-water mixture was stored, the 0,24,72,144,216,288 hours on sperm motility, sperm hyposmotic expansion and spermopia Body integrity testing. The results showed that the addition of trehalose at concentrations below 20 mmol / dm 3 in TRIS semen dilution did not increase the sperm motility of the preserved semen, but significantly increased the acrosome intact sperm integrity and the optimal trehalose additive The amount is 5 mmol / dm ~ 3.