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WRKY是植物体内最大的转录因子家族之一,其成员在植物生长发育和抗逆过程中发挥重要作用。为了解水稻WRKY的功能,本研究利用蛋白质免疫印迹(w estern blotting,WB)技术,检测了8个WRKY转录因子在水稻叶片生长和Xa21介导的抗白叶枯病过程中的表达丰度变化。结果表明:OsWRKY12、43、55和86在苗期表达量较低,随叶片的生长表达逐步增加;OsWRKY50和84在苗期叶片中的表达量相对较高,成熟期下降;未检测到OsWRKY40/64和52在叶片中的表达。在Xa21介导的白叶枯病抗性反应中,OsWRKY40/64、50和52受白叶枯病菌诱导表达,OsWRKY84受侵染后表达量上调,OsWRKY12、43、55和86在抗病过程中没有检测到表达丰度的变化。进一步比较这些转录因子在抗、感和对照(Mock)反应中的表达,发现抗、感反应间蛋白质的表达变化是相似的。研究结果提示:OsWRKY12、43、55和86可能在叶片正常生长中发挥作用;OsWRKY40/64和52可能在水稻-白叶枯病菌互作过程中发挥作用;OsWRKY50和84则在叶片生长和抗白叶枯病过程中都有一定的功能。
WRKY is one of the largest family of transcription factors in plants and its members play an important role in plant growth and development. In order to understand the function of WRKY in rice, we used Western blotting (WB) to detect the expression abundance of eight WRKY transcription factors in rice leaf growth and Xa21-mediated bacterial leaf blight resistance . The results showed that the expression levels of OsWRKY12, 43, 55 and 86 were lower at seedling stage and gradually increased with the growth of leaves. The expression levels of OsWRKY50 and 84 in leaves at seedling stage were relatively high and decreased at maturity stage. OsWRKY40 / Expression of 64 and 52 in leaves. In Xa21-mediated bacterial leaf blight resistance, OsWRKY40 / 64, 50 and 52 were induced by the bacterial blight, OsWRKY84 was up-regulated after infection, and OsWRKY12, 43, 55 and 86 were resistant to the disease No change in expression abundance was detected. Further comparing the expression of these transcription factors in the anti-sense and Mock responses, we found that the expression changes of the anti-sense and anti-sense proteins are similar. The results indicated that OsWRKY12, 43, 55 and 86 may play roles in the normal growth of leaves. OsWRKY40 / 64 and 52 may play roles in the interaction between rice and bacterial blight. OsWRKY50 and 84 play an important role in leaf growth and anti-white Leaf blight process has a certain function.