Inokosterone Is a Potential Drug Target of Estrogen Receptor 1 in Rheumatoid Arthritis Patients:Anal

• 作 者: MO Ji-hao XlE Han-kun ZHOU Ye-mian Ng Sihan-benjamin LI Shao-xia WANG Lei
• 机 构: Department of Medical Laboratory,Luoyang Orthopedic Hospital of Henan Province,Orthopedic Institute
• 出 处: 中国结合医学杂志（英文版）
• 发表日期: 2021年10期
• 关键词: rheumatoid arthritis estrogen receptor 1 inokosterone Cyathula Officinalis Chine

Objective:To elucidate the active compounds and the molecular mechanism of Cyathula Officinalis as a drug treatment for rheumatoid arthritis (RA).Methods:The target genes of active ingredients from Cyathula Officinalis were obtained from bioinformatics analysis tool for the molecular mechanism of traditional Chinese medicine.The protein-protein interaction between the target genes were analyzed using STRING and Genemania.The transcriptome of RA patients compared to healthy people (GSE121894) were analyzed using R program package Limma.The relative expression of the target genes was obtained from the RNA-seq datasets.The molecular docking analyses were processed based on the molecular model of estrogen receptor 1 (ESR1) binding with estradiol (PDB ID:1A52).The binding details were analyzed by SYBYL.Results:Inokosterone,ecdysterone,and cyaterone were the 3 active ingredients from Cyathula Officinalis that bind to target genes.Of all the significantly changed genes from RA patients,ESR1,ADORA1,and ANXA1 were significantly increased in mRNA samples of RA patients.Conclusion:ESR1,the transcription factor that binds inokosterone in the molecular binding analysis,is the target protein of Cyathula Officinalis.